Overview

  • Product name

    Human CXCL9 ELISA Kit
    See all CXCL9 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 3 3.4%
    Inter-assay
    Sample n Mean SD CV%
    Overall 5 5.9%
  • Sample type

    Cell culture supernatant, Serum, Cell culture extracts, Tissue Extracts, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    5 pg/ml
  • Range

    28.13 pg/ml - 1800 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 90 86% - 92%
    Serum 115 103% - 131%
    Tissue Extracts 109 104% - 116%
    EDTA Plasma 109 99% - 125%
    Citrate Plasma 119 110% - 127%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Human CXCL9 SimpleStep ELISA® kit (ab176108) has been re-developed with new capture and detector antibodies. This new kit has the same name but a different product number (ab219047). We have identified new recombinant monoclonal antibodies to use in the SimpleStep ELISA platform that provide a higher sensitivity when quantifying MIG in human serum, citrate plasma, EDTA plasma, cell culture extracts and tissue extracts.


    CXCL9 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CXCL9 protein in human serum, plasma, cell culture supernatants, and cell and tissue extracts.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Sensitivity:


    Samples in Sample Diluent NS: 5.0 pg/mL
    Samples in 1X Cell Extraction Buffer PTR: 16.6 pg/mL
    Samples in Sample Diluent 10BP: 14.6 pg/mL

  • Notes

    CXCL9, is a small cytokine belonging to the CXC chemokine subfamily that lacks an ELR motif in front of the first cysteine. CXCL9, also known as MIG,  (Monokine Induced by Gamma Interferon) is a T-cell chemoattractant, which is induced by Interferon Gamma. This subfamily also includes Interferon Gamma Induced Protein 10 (IP-10 or CXCL10) and Interferon Inducible T-cell Alpha Chemoattractant (I-TAC or CXCL11) whose genes are located near the gene for MIG on human chromosome 4. MIG, IP-10 and I-TAC all elicit their chemotactic functions by interacting with the G protein coupled chemokine receptor CXCR3 (GPR9 or CD183).

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human CXCL9 Capture Antibody 1 x 600µl
    10X Human CXCL9 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 4BI 1 x 6ml
    Human CXCL9 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent 25BP 1 x 20ml
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    Cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. Chemotactic for activated T-cells. Binds to CXCR3.
  • Sequence similarities

    Belongs to the intercrine alpha (chemokine CxC) family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • C-X-C motif chemokine 9
    • chemokine (C-X-C motif) ligand 9
    • CMK
    • crg-10
    • CXCL9
    • CXCL9_HUMAN
    • gamma interferon induced monokine
    • Gamma-interferon-induced monokine
    • HuMIG
    • MIG
    • monokine induced by gamma interferon
    • monokine induced by interferon gamma
    • Monokine induced by interferon-gamma
    • SCYB9
    • Small inducible cytokine B9
    • small inducible cytokine subfamily B member 9
    • Small-inducible cytokine B9
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab219047 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Standard curve comparison between human MIG SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of MIG were measured in duplicates, interpolated from the MIG standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100% (neat), plasma (citrate) 100% (neat), and plasma (EDTA) 100% (neat). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • The concentrations of MIG were measured in duplicates, interpolated from the MIG standard curves and corrected for sample dilution. Undiluted samples are as follows: PHA-M stimulated PBMC supernatant 2.5% and unstimulated PBMC supernatant 100% (neat). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MIG concentration was determined to be 20.4 ng/mL in neat PHA-M stimulated PBMC supernatant and 1.62 ng/mL in neat unstimulated PBMC supernatant.

  • The concentrations of MIG were measured in duplicate and interpolated from the MIG standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MIG concentration was determined to be 993.9 pg/mL in PHA-M stimulated PBMC cell extract and 824.9 pg/mL in thyroid tissue extract.

  • Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean of detectable MIG concentration was determined to be 90.2 pg/mL with a range of 47.8 – 149 pg/mL. Detectable concentrations are defined as concentration above the lowest standard concentration.

  • Human PBMC cells were cultured in the absence or presence of 1.5% PHA-M for 2 days. The concentrations of MIG were measured in three different dilutions of the supernatant samples in duplicates and interpolated from the MIG standard curve. The interpolated values are plotted (mean +/- SD, n=2). The mean MIG concentration was determined to be 20.4 pg/mL in PHA-M stimulated PBMC cell supernatant, 1.6 pg/mL in unstimulated supernatants and undetectable in media (not shown).

Protocols

References

ab219047 has not yet been referenced specifically in any publications.

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