Overview

  • Product name

    Human CYR61 Antibody Pair - BSA and Azide free
    See all CYR61 kits
  • Detection method

    Colorimetric
  • Assay type

    ELISA set
  • Range

    31.3 pg/ml - 3000 pg/ml
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Antibody Pair can be used to quantify Human CYR61. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.


    For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab238267), which uses the same antibodies. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.


    To receive an electronic copy of the Certificate of Analysis, please send an email to technical support with "CoA for matched antibody pair kit" in the subject line and the desired product number and lot number in the body of the email.


    Download SDS here.


     

  • Tested applications

    Suitable for: ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 10 x 96 tests
    Human CYR61 Capture Antibody 1 x 100µg
    Human CYR61 Detector Antibody 1 x 100µg
  • Research areas

  • Relevance

    CYR61 is a secreted, cysteine rich, heparin binding protein encoded by a growth factor inducible immediate early gene. It promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion, and appears to play a role in wound healing by up regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA A, VEGA C, MMP1, MMP3, TIMP1, uPA, PAI 1 and integrins alpha 3 and alpha 5. Cyr61 mediated gene regulation is dependent on heparin binding. CYR61 down regulates the expression of alpha 1 and alpha 2 subunits of collagen type 1. It promotes cell adhesion and adhesive signaling through integrin alpha 6/beta 1, cell migration through integrin alpha v/beta 5 and cell proliferation through integrin alpha v/beta 3.
  • Cellular localization

    Secreted
  • Alternative names

    • CCN1
    • CYR61 protein precursor
    • Cysteine rich angiogenic inducer 61
    • GIG1 protein
    • IGFBP10
    • Insulin like growth factor binding protein 10
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab244159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

References

ab244159 has not yet been referenced specifically in any publications.

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