Human CYR61 ELISA Kit (ab238267)
- Datasheet
- References
- Protocols
Overview
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Product name
Human CYR61 ELISA Kit
See all CYR61 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Supernatant 5 2.5% Inter-assay Sample n Mean SD CV% Supernatant 3 3.8% -
Sample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Cell culture extracts, Tissue Extracts, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
5 pg/ml -
Range
31.3 pg/ml - 3000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 100 94% - 103% Saliva 114 110% - 119% Milk 95 93% - 96% Urine 107 103% - 113% Serum 112 108% - 115% Cell culture extracts 83 80% - 85% Tissue Extracts 84 80% - 88% Cell culture media 91 89% - 96% EDTA Plasma 85 84% - 86% Cit plasma 92 86% - 96% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Cow -
Product overview
CYR61 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CYR61 protein in human serum, plasma, milk, urine, saliva, cell culture supernatants, and cell and tissue extracts.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB Development Solution is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
CYR61 (Cysteine-rich Angiogenic Inducer 61), also known as CCN Family Member 1(CCN1) or Insulin-Like Growth Factor-Binding Protein 10 (IGFBP10) is a secreted protein that promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. CYR61 exhibits both tumorigenic and tumor suppressor properties, depending in part on the localization.
Sensitivity:
Samples in Sample Diluent NS: 5.0 pg/mL.
Samples in Sample Diluent 25BP: 7.1 pg/mL.
Samples in 1X Cell Extraction Buffer PTR: 6.6 pg/mL.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human CYR61 Capture Antibody 1 x 600µl 10X Human CYR61 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BI 1 x 6ml Human CYR61 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 25BP 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Relevance
CYR61 is a secreted, cysteine rich, heparin binding protein encoded by a growth factor inducible immediate early gene. It promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion, and appears to play a role in wound healing by up regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA A, VEGA C, MMP1, MMP3, TIMP1, uPA, PAI 1 and integrins alpha 3 and alpha 5. Cyr61 mediated gene regulation is dependent on heparin binding. CYR61 down regulates the expression of alpha 1 and alpha 2 subunits of collagen type 1. It promotes cell adhesion and adhesive signaling through integrin alpha 6/beta 1, cell migration through integrin alpha v/beta 5 and cell proliferation through integrin alpha v/beta 3. -
Cellular localization
Secreted -
Alternative names
- CCN1
- CYR61 protein precursor
- Cysteine rich angiogenic inducer 61
see all -
Database links
- Entrez Gene: 3491 Human
- Omim: 602369 Human
- SwissProt: O00622 Human
- SwissProt: Q9UID7 Human
- SwissProt: Q6FI18 Human
- Unigene: 8867 Human
Associated products
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Alternative Versions
Applications
Our Abpromise guarantee covers the use of ab238267 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Other - Human CYR61 ELISA Kit (ab238267)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human CYR61 Standard Curve Comparison.Standard Curve comparison between Human CYR61 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity. -
Example of human CYR61 standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Example of human CYR61 standard curve in Sample Diluent 25BP.Background-subtracted data values (mean +/- SD) are graphed.
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Example of human CYR61 standard curve in 1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native CYR61 in human serum and plasma samples.The concentrations of CYR61 were measured in duplicates, interpolated from the CYR61 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100%, plasma (citrate) 100%, and plasma (EDTA) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CYR61 concentration was determined to be 182.2 pg/mL in neat serum, 123.5 pg/mL in neat plasma (citrate), and 155.3 pg/mL in neat plasma (EDTA). -
Interpolated concentrations of native CYR61 in human breast milk (de-fatted), saliva, and cell culture supernatant (SN) samples.The concentrations of CYR61 were measured in duplicates, interpolated from the CYR61 standard curves and corrected for sample dilution. Undiluted samples are as follows: milk 100%, saliva 100%, HeLa supernatant 25%, and MDA-MB-453S 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CYR61 concentration was determined to be 1,432 pg/mL in neat milk, 798.2 pg/mL in neat saliva, 8,357 pg/mL neat HeLa cell culture supernatant, and 1,984 pg/mL in neat MDA-MB-453S cell culture supernatant.
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Interpolated concentrations of native CYR61 in human HeLa cell extract based on a 250 µg/mL extract load and MDA-MB435S cell extract based on a 500 µg/mL extract load.The concentrations of CYR61 were measured in duplicate and interpolated from the CYR61 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CYR61 concentration was determined to be 1,097 pg/mL in HeLa cell extract and 1,717 pg/mL in MDA-MB-435S cell extract. -
Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CYR61 concentration was determined to be 170.3 pg/mL with a range of 97. 07 – 254.9 pg/mL.
Protocols
References
ab238267 has not yet been referenced specifically in any publications.
