Human CYR61/CCN1 ELISA Kit (ab213772)

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Overview

  • Product name

    Human CYR61/CCN1 ELISA Kit
    See all CYR61/CCN1 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 16 628pg/ml 19.5 3.1%
    2 16 1145pg/ml 36.6 3.2%
    3 16 1574pg/ml 53.5 3.4%
    Inter-assay
    Sample n Mean SD CV%
    1 24 570pg/ml 27.93 4.9%
    2 24 1097pg/ml 58.1 5.3%
    3 24 1563pg/ml 96.9 6.2%

  • Sample type

    Cell culture supernatant, Saliva, Milk, Urine, Serum, Hep Plasma, EDTA Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    < 10 pg/ml

  • Range

    31.2 pg/ml - 2000 pg/ml

  • Assay time

    3h 30m

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    The Human CYR61/CCN1 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213772) is designed for the quantitative measurement of Human CYR61/CCN1 in cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and human milk.


    The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for CYR61/CCN1 has been pre-coated onto 96-well plates. Standards (Expression system for standard: CHO; Immunogen sequence: A22-D381) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CYR61/CCN1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human CYR61/CCN1 amount of sample captured in plate.

  • Notes

    Cysteine-rich angiogenic inducer 61 (CYR61) or CCN family member 1 (CCN1), is a matricellular protein that in humans is encoded by the CYR61 gene. This gene is mapped to 1p22.3. CYR61 is capable of regulating a broad range of cellular activities, including cell adhesion, migration, proliferation, differentiation, apoptosis and senescence through interaction with cell surface integrin receptors and heparan sulfate proteoglycans. During embryonic development, CYR61 is critical for cardiac septal morphogenesis, blood vessel formation in placenta and vascular integrity. In adulthood CYR61 plays important roles in inflammation and tissue repair and is associated with diseases related to chronic inflammation, including rheumatoid arthritis, atherosclerosis, diabetes related nephropathy and retinopathy, and many different forms of cancers.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-Human CYR61/CCN1 coated Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti- Human CYR61/CCN1 antibody 1 x 130µl
    Lyophilized recombinant Human CYR61/CCN1 standard 2 vials
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Black Cap 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml

  • Research areas

  • Function

    Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3.

  • Sequence similarities

    Belongs to the CCN family.
    Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 1 IGFBP N-terminal domain.
    Contains 1 TSP type-1 domain.
    Contains 1 VWFC domain.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Alternative names

    • 3CH61
    • CCN 1
    • CCN family member 1
    • CCN1
    • CYR 61
    • Cyr61
    • CYR61 protein
    • CYR61_HUMAN
    • Cysteine rich angiogenic inducer 61
    • Cysteine rich heparin binding protein 61
    • Cysteine-rich angiogenic inducer 61
    • GIG 1
    • GIG1
    • IBP-10
    • IGF-binding protein 10
    • Igfbp 10
    • IGFBP-10
    • Igfbp10
    • Insulin like growth factor binding protein 10
    • Insulin-like growth factor-binding protein 10
    • Protein CYR61
    • Protein GIG1
    see all

  • Database links

    Images

    • Human CYR61 ELISA Kit (CCN1) (ab213772) Standard Curve.
      Human CYR61 ELISA Kit (CCN1) (ab213772) Standard Curve.

      Human CYR61 ELISA Kit (CCN1) (ab213772) Standard Curve.

    Protocols

    References

    ab213772 has not yet been referenced specifically in any publications.

    Publishing research using ab213772? Please let us know so that we can cite the reference in this datasheet.

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