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Cardiovascular Angiogenesis Adhesion / ECM Integrins
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Human CYR61/CCN1 ELISA Kit (ab213772)

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  • SDS
  • Protocol Booklet
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Human CYR61 ELISA Kit (CCN1) (ab213772) Standard Curve.

    Key features and details

    • Sensitivity: 10 pg/ml
    • Range: 31.2 pg/ml - 2000 pg/ml
    • Sample type: Cell culture supernatant, EDTA Plasma, Hep Plasma, Milk, Saliva, Serum, Urine
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Human

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    Recombinant human CYR61/CCN1 protein (Active) (ab50074)

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    Overview

    • Product name

      Human CYR61/CCN1 ELISA Kit
      See all CYR61/CCN1 kits
    • Detection method

      Colorimetric
    • Precision

      Intra-assay
      Sample n Mean SD CV%
      1 16 61pg/ml 2.86 = 4.7%
      2 16 248pg/ml 16.12 = 6.5%
      3 16 814pg/ml 55.35 = 6.8%
      Inter-assay
      Sample n Mean SD CV%
      1 24 59pg/ml 3.3 = 5.6%
      2 24 242pg/ml 17.66 = 7.3%
      3 24 806pg/ml 64.48 = 8%
    • Sample type

      Cell culture supernatant, Saliva, Milk, Urine, Serum, Hep Plasma, EDTA Plasma
    • Assay type

      Sandwich (quantitative)
    • Sensitivity

      < 10 pg/ml
    • Range

      31.2 pg/ml - 2000 pg/ml
    • Assay time

      3h 30m
    • Assay duration

      Multiple steps standard assay
    • Species reactivity

      Reacts with: Human
    • Product overview

      The Human CYR61/CCN1 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213772) is designed for the quantitative measurement of Human CYR61/CCN1 in cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and human milk.


      The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for CYR61/CCN1 has been pre-coated onto 96-well plates. Standards (Expression system for standard: CHO; Immunogen sequence: A22-D381) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CYR61/CCN1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human CYR61/CCN1 amount of sample captured in plate.

    • Notes

      Cysteine-rich angiogenic inducer 61 (CYR61) or CCN family member 1 (CCN1), is a matricellular protein that in humans is encoded by the CYR61 gene. This gene is mapped to 1p22.3. CYR61 is capable of regulating a broad range of cellular activities, including cell adhesion, migration, proliferation, differentiation, apoptosis and senescence through interaction with cell surface integrin receptors and heparan sulfate proteoglycans. During embryonic development, CYR61 is critical for cardiac septal morphogenesis, blood vessel formation in placenta and vascular integrity. In adulthood CYR61 plays important roles in inflammation and tissue repair and is associated with diseases related to chronic inflammation, including rheumatoid arthritis, atherosclerosis, diabetes related nephropathy and retinopathy, and many different forms of cancers.

    • Platform

      Pre-coated microplate (12 x 8 well strips)

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components Identifier 1 x 96 tests
      ABC Diluent Buffer Blue Cap 1 x 12ml
      Adhesive Plate Seal 4 units
      Antibody Diluent Buffer Green Cap 1 x 12ml
      Anti-Human CYR61/CCN1 coated Microplate (12 x 8 wells) 1 unit
      Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl
      Biotinylated anti- Human CYR61/CCN1 antibody 1 x 130µl
      Lyophilized recombinant Human CYR61/CCN1 standard 2 vials
      Sample Diluent Buffer Green Cap 1 x 30ml
      TMB Color Developing Agent Black Cap 1 x 10ml
      TMB Stop Solution Yellow Cap 1 x 10ml
    • Research areas

      • Cardiovascular
      • Angiogenesis
      • Adhesion / ECM
      • Integrins
      • Cardiovascular
      • Angiogenesis
      • Adhesion / ECM
      • Extracellular Matrix
      • Immunology
      • Secreted Molecules
      • CCN Family
      • Stem Cells
      • Mesenchymal Stem Cells
      • Secreted Molecules
    • Function

      Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3.
    • Sequence similarities

      Belongs to the CCN family.
      Contains 1 CTCK (C-terminal cystine knot-like) domain.
      Contains 1 IGFBP N-terminal domain.
      Contains 1 TSP type-1 domain.
      Contains 1 VWFC domain.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession O00622 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • 3CH61
      • CCN 1
      • CCN family member 1
      • CCN1
      • CYR 61
      • Cyr61
      • CYR61 protein
      • CYR61_HUMAN
      • Cysteine rich angiogenic inducer 61
      • Cysteine rich heparin binding protein 61
      • Cysteine-rich angiogenic inducer 61
      • GIG 1
      • GIG1
      • IBP-10
      • IGF-binding protein 10
      • Igfbp 10
      • IGFBP-10
      • Igfbp10
      • Insulin like growth factor binding protein 10
      • Insulin-like growth factor-binding protein 10
      • Protein CYR61
      • Protein GIG1
      see all
    • Database links

      • Entrez Gene: 3491 Human
      • Omim: 602369 Human
      • SwissProt: O00622 Human
      • Unigene: 8867 Human

      Associated products

        Images

        • Human CYR61 ELISA Kit (CCN1) (ab213772) Standard Curve.
          Human CYR61 ELISA Kit (CCN1) (ab213772) Standard Curve.

          Human CYR61 ELISA Kit (CCN1) (ab213772) Standard Curve.

        Protocols

        • Protocol Booklet

        Click here to view the general protocols

        Datasheets and documents

        • Datasheet
        • SDS
      • References (0)

        Publishing research using ab213772? Please let us know so that we can cite the reference in this datasheet.

        ab213772 has not yet been referenced specifically in any publications.

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