Human CYR61/CCN1 ELISA Kit (ab213772)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 31.2 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, EDTA Plasma, Hep Plasma, Milk, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human CYR61/CCN1 ELISA Kit
See all CYR61/CCN1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 1 16 61pg/ml 2.86 = 4.7% 2 16 248pg/ml 16.12 = 6.5% 3 16 814pg/ml 55.35 = 6.8% Inter-assay Sample n Mean SD CV% 1 24 59pg/ml 3.3 = 5.6% 2 24 242pg/ml 17.66 = 7.3% 3 24 806pg/ml 64.48 = 8% -
Sample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 10 pg/ml -
Range
31.2 pg/ml - 2000 pg/ml -
Assay time
3h 30m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
The Human CYR61/CCN1 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213772) is designed for the quantitative measurement of Human CYR61/CCN1 in cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and human milk.
The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for CYR61/CCN1 has been pre-coated onto 96-well plates. Standards (Expression system for standard: CHO; Immunogen sequence: A22-D381) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CYR61/CCN1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human CYR61/CCN1 amount of sample captured in plate.
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Notes
Cysteine-rich angiogenic inducer 61 (CYR61) or CCN family member 1 (CCN1), is a matricellular protein that in humans is encoded by the CYR61 gene. This gene is mapped to 1p22.3. CYR61 is capable of regulating a broad range of cellular activities, including cell adhesion, migration, proliferation, differentiation, apoptosis and senescence through interaction with cell surface integrin receptors and heparan sulfate proteoglycans. During embryonic development, CYR61 is critical for cardiac septal morphogenesis, blood vessel formation in placenta and vascular integrity. In adulthood CYR61 plays important roles in inflammation and tissue repair and is associated with diseases related to chronic inflammation, including rheumatoid arthritis, atherosclerosis, diabetes related nephropathy and retinopathy, and many different forms of cancers.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Adhesive Plate Seal 4 units Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Human CYR61/CCN1 coated Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti- Human CYR61/CCN1 antibody 1 x 130µl Lyophilized recombinant Human CYR61/CCN1 standard 2 vials Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Black Cap 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml -
Research areas
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Function
Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3. -
Sequence similarities
Belongs to the CCN family.
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 1 IGFBP N-terminal domain.
Contains 1 TSP type-1 domain.
Contains 1 VWFC domain. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- 3CH61
- CCN 1
- CCN family member 1
see all -
Database links
- Entrez Gene: 3491 Human
- Omim: 602369 Human
- SwissProt: O00622 Human
- Unigene: 8867 Human
Associated products
Images
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Human CYR61 ELISA Kit (CCN1) (ab213772) Standard Curve.
Human CYR61 ELISA Kit (CCN1) (ab213772) Standard Curve.
References (0)
ab213772 has not yet been referenced specifically in any publications.
