RecombinantRabMAb

Human Cytokeratin 18 Antibody Pair - BSA and Azide free (ab244043)

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Sandwich ELISA - Human Cytokeratin 18 Antibody Pair - BSA and Azide free (ab244043)

    Key features and details

    • Unconjugated capture and detector antibodies
    • Adaptable to any antibody pair-based assay format
    • Antibody concentration ~ 1 mg/ml
    • BSA and azide free buffer - ready for conjugation
    • Reacts with: Human

    Overview

    • Product name

      Human Cytokeratin 18 Antibody Pair - BSA and Azide free
      See all Cytokeratin 18 kits

    • Assay type

      ELISA set

    • Range

      15.6 pg/ml - 1000 pg/ml

    • Species reactivity

      Reacts with: Human

    • Product overview

      The Antibody Pair can be used to quantify Human Cytokeratin 18. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.


      For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab227896), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.


      Download SDS here.

    • Tested applications

      Suitable for: Sandwich ELISAmore details

    • Platform

      Reagents

    Properties

    • Storage instructions

      Store at +4°C. Please refer to protocols.

    • Carrier free

      Yes
    • Components 10 x 96 tests
      Human Cytokeratin 18 Capture Antibody (unconjugated) 1 x 100µg
      Human Cytokeratin 18 Detector Antibody (unconjugated) 1 x 100µg

    • Research areas

    • Function

      Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

    • Tissue specificity

      Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.

    • Involvement in disease

      Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].

    • Sequence similarities

      Belongs to the intermediate filament family.

    • Post-translational
      modifications

      Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
      Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
      O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.

    • Cellular localization

      Cytoplasm > perinuclear region.
    • Information by UniProt

    • Alternative names

      • Cell proliferation inducing gene 46 protein
      • Cell proliferation inducing protein 46
      • Cell proliferation-inducing gene 46 protein
      • CK 18
      • CK-18
      • CK18
      • CYK 18
      • CYK18
      • Cytokeratin 18
      • Cytokeratin endo B
      • Cytokeratin-18
      • K 18
      • K18
      • K1C18_HUMAN
      • KA18
      • Keratin 18
      • Keratin 18, type I
      • Keratin D
      • keratin, type I cytoskeletal 18
      • Keratin-18
      • Krt18
      see all

    • Database links

      Associated products

      Applications

      Our Abpromise guarantee covers the use of ab244043 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Abreviews Notes
      Sandwich ELISA Use at an assay dependent concentration.

      Images

      • Sandwich ELISA - Human Cytokeratin 18 Antibody Pair - BSA and Azide free (ab244043)
        Sandwich ELISA - Human Cytokeratin 18 Antibody Pair - BSA and Azide free (ab244043)
        To learn more about the advantages of recombinant antibodies see here.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab244043? Please let us know so that we can cite the reference in this datasheet.

      ab244043 has not yet been referenced specifically in any publications.

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      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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