Human Cytokine Antibody Array (80 Targets) - Quantitative (ab197425)

Overview

  • Product name

    Human Cytokine Antibody Array (80 Targets) - Quantitative
  • Sample type

    Cell culture supernatant, Serum, Plasma, Other biological fluids, Cell Lysate, Tissue Lysate
  • Assay type

    Quantitative
  • Assay time

    6h 00m
  • Species reactivity

    Reacts with: Human
  • Product overview

    ab197425 can be used for quantitative measurement of 80 Human cytokines. Suitable for serum, plasma, cell culture media, other body fluid, cell and tissue lysates.
    Targets: BLC, Eotaxin, Eotaxin-2, G-CSF, GM-CSF, I-309, ICAM-1, IFNγ, IL-1α, IL-1β, IL-1ra, IL-2, IL-4, IL-5, IL-6, IL-6 sR, IL-7, IL-8, IL-10, IL-11, IL-12p40, IL-12p70, IL-13, IL-15, IL-16, IL-17, MCP-1, M-CSF, MIG, MIP-1α, MIP-1β, MIP-1δ, PDGF-BB, RANTES, TIMP-1, TIMP-2, TNFα, TNFβ, TNF sRI, TNF sRII, AR, BDNF, bFGF, BMP-4, BMP-5, BMP-7, β-NGF, EGF, EGF R, EG-VEGF, FGF-4, FGF-7, GDF-15, GDNF, GH, HB-EGF, HGF, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, IGF-I, Insulin, MCSF R, NGF R, NT-3, NT-4, OPG, PDGF-AA, PIGF, SCF, SCF R, TGFα, TGFβ1, TGFβ3, VEGF, VEGF R2, VEGF R3, VEGF-D. 

    The analytes measured by this kit are split across 2 different arrays on different slides, both of which are provided in the kit. 

    Learn more about Quantitative antibody arrays:

  • Notes

    Quantitative antibody arrays can be used to quantitate up to 40 cytokines with as little as 50 µL of sample. Arrays are available for 400 human and 200 mouse proteins. 

    Each glass slide is spotted with 16 identical antibody arrays and is provided with a 16 well gasket to allow separate samples to be applied to each array. Each antibody is spotted in quadruplicate in each array. For reproducible quantitation, eight of the arrays are used with a cocktail of protein standards to produce a standard curve. The same 8 standard curve arrays can be used across multiple slides, allowing measurement of 22 experimental samples with 2 slides, 36 with 3 slides etc. 

    For high through-put, 4 slides can be nested into a tray matching a standard microplate, allowing automated processing with a liquid handling workstation. 

    Array processing can be completed within one working day (see workflow diagram below) and arrays can be analyzed with a wide number of laser glass slide array / gene microarray scanners. If you don’t have a suitable scanner then we recommend our membrane antibody arrays, which can be analyzed with any WB chemiluminescent reader. 

    Learn more about quantitative antibody arrays

    Learn more about membrane antibody arrays.

Properties

Images

  • Human Cytokine Antibody Array - Quantitative (80 Targets) (ab19725) Assay Summary

Protocols

References

ab197425 has not yet been referenced specifically in any publications.

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