Product nameHuman Cytokine Antibody Array (80 Targets) - Quantitative
Sample typeCell culture supernatant, Serum, Plasma, Other biological fluids, Cell Lysate, Tissue Lysate
Assay time6h 00m
Species reactivityReacts with: Human
ab197425 can be used for quantitative measurement of 80 Human cytokines. Suitable for serum, plasma, cell culture media, other body fluid, cell and tissue lysates.
Targets: BLC, Eotaxin, Eotaxin-2, G-CSF, GM-CSF, I-309, ICAM-1, IFNγ, IL-1α, IL-1β, IL-1ra, IL-2, IL-4, IL-5, IL-6, IL-6 sR, IL-7, IL-8, IL-10, IL-11, IL-12p40, IL-12p70, IL-13, IL-15, IL-16, IL-17, MCP-1, M-CSF, MIG, MIP-1α, MIP-1β, MIP-1δ, PDGF-BB, RANTES, TIMP-1, TIMP-2, TNFα, TNFβ, TNF sRI, TNF sRII, AR, BDNF, bFGF, BMP-4, BMP-5, BMP-7, β-NGF, EGF, EGF R, EG-VEGF, FGF-4, FGF-7, GDF-15, GDNF, GH, HB-EGF, HGF, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, IGF-I, Insulin, MCSF R, NGF R, NT-3, NT-4, OPG, PDGF-AA, PIGF, SCF, SCF R, TGFα, TGFβ1, TGFβ3, VEGF, VEGF R2, VEGF R3, VEGF-D.
The analytes measured by this kit are split across 2 different arrays on different slides, both of which are provided in the kit.
Quantitative antibody arrays can be used to quantitate up to 40 cytokines with as little as 50 µL of sample. Arrays are available for 400 human and 200 mouse proteins.
Each glass slide is spotted with 16 identical antibody arrays and is provided with a 16 well gasket to allow separate samples to be applied to each array. Each antibody is spotted in quadruplicate in each array. For reproducible quantitation, eight of the arrays are used with a cocktail of protein standards to produce a standard curve. The same 8 standard curve arrays can be used across multiple slides, allowing measurement of 22 experimental samples with 2 slides, 36 with 3 slides etc.
For high through-put, 4 slides can be nested into a tray matching a standard microplate, allowing automated processing with a liquid handling workstation.
Array processing can be completed within one working day (see workflow diagram below) and arrays can be analyzed with a wide number of laser glass slide array / gene microarray scanners. If you don’t have a suitable scanner then we recommend our membrane antibody arrays, which can be analyzed with any WB chemiluminescent reader.
The standard is affinity purified.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 8 tests 22 tests 50 tests 20X Wash Buffer I 3 x 30ml 6 x 30ml 12 x 30ml 20X Wash Buffer II 1 x 30ml 2 x 30ml 4 x 30ml Cy3 equivalent dye-conjugated Streptavidin 2 x 5µl 4 x 5µl 8 x 5µl Human Growth Factor Array (40 T) Biotinylated Antibody Cocktail 1 unit 2 units 4 units Human Growth Factor Array (40 T) Glass Slide 1 slide 2 slides 4 slides Human Growth Factor Array (40 T) Lyophilized Cytokine Standard Mix 1 vial 2 vials 2 vials Human Inflammation Array (40 T) Biotinylated Antibody Cocktail 1 unit 2 units 4 units Human Inflammation Array (40 T) Glass Slide 1 slide 2 slides 4 slides Human Inflammation Array (40 T) Lyophilized Cytokine Standard Mix 1 vial 2 vials 2 vials Sample Diluent 1 x 15ml 2 x 15ml 4 x 15ml Slide Washer/Dryer (30 mL Centrifuge Tube) 1 unit 2 units 4 units
Human Cytokine Antibody Array - Quantitative (80 Targets) (ab19725) Assay Summary
ab197425 has not yet been referenced specifically in any publications.