Human Cytokine Antibody Array II (Membrane, 32 Targets) (ab211060)

Overview

  • Product name

    Human Cytokine Antibody Array II (Membrane, 32 Targets)
  • Sample type

    Serum, Plasma, Other biological fluids, Cell Lysate, Cell culture media, Tissue Lysate
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Human Cytokine Antibody Array II (ab211060) for use with cell cultured media, serum, plasma, cell and tissue lysates and other liquid samples.


    Targets: aFgF (FGF-1), Albumin, AMICA, ANG-4, BAFF, CD163, Clusterin, CRTAM, CXCL14 (BRAK4), Cystatin C, DLL1, Fetuin A, FOLR1, GASP-1, GASP-2, GCSF R (CD114), HAI-2, IL-17 RB, IL-27, LAG-3, LDL R, Pepsinogen I, RBP4, SOST, Syndecan-1, TACI (TNFRSF13B), TFPI, Thrombospondin-1, TRANCE (TNFSF11), Troponin 1, uPA, WISP-1 (CCN4).

  • Notes

    Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1 mL of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.

    Learn more about cytokine arrays and other membrane antibody arrays

  • Tested applications

    Suitable for: Multiplex Protein Detectionmore details

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 2 Membrane 4 Membrane 8 Membrane
    1,000X HRP-Streptavidin Buffer 1 x 50µl 1 x 50µl 1 x 50µl
    20X Wash Buffer I 1 x 10ml 1 x 10ml 1 x 20ml
    20X Wash Buffer II 1 x 10ml 1 x 10ml 1 x 20ml
    2X Cell Lysis Buffer 1 x 10ml 1 x 10ml 1 x 16ml
    8-Well Incubation Tray (with Lid) 1 unit 1 unit 1 unit
    Antibody Arrays 2 units 4 units 8 units
    Biotinylated Antibody Cocktail 1 vial 2 vials 4 vials
    1X Blocking Buffer 1 x 25ml 1 x 25ml 2 x 25ml
    Detection Buffer C 1 x 1.5ml 1 x 1.5ml 1 x 2.5ml
    Detection Buffer D 1 x 1.5ml 1 x 1.5ml 1 x 2.5ml
    Plastic sheets 1 unit 1 unit 1 unit

Applications

Our Abpromise guarantee covers the use of ab211060 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Multiplex Protein Detection Use at an assay dependent concentration.

Images

  • Membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at room temperature for 1 minute.

Protocols

References

ab211060 has not yet been referenced specifically in any publications.

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