Overview

  • Product name

    Human Cytokine Antibody Array - Membrane (120 Targets)
    See all Cytokine Antibody Array antibody arrays
  • Sample type

    Serum, Plasma, Other biological fluids, Cell Lysate, Cell culture media, Tissue Lysate
  • Assay type

    Semi-quantitative
  • Species reactivity

    Reacts with: Human
  • Product overview

    ab193656 can be used for the simultaneous detection of 120 Human Cytokine proteins in serum, plasma, cell culture media and other liquid samples types. It comes as 1 kit including 2 separate membranes labelled C6 and C7 (see below for images showing the array layout on each membrane).”
    Targets names: Adiponectin (ACRP30), AgRP, Amphiregulin, Angiogenin, Angiopoietin-2, Axl, BDNF, bFGF, BLC (CXCL13), BMP-4, BMP-6, beta-NGF, Betacellulin (BTC), CCL28 (MEC), CK beta 8-1 (CCL23), CNTF, CTACK (CCL27), Dtk, EGF, EGFR, ENA-78 (CXCL5), Eotaxin-1 (CCL11), Eotaxin-2 (MPIF-2/CCL24), Eotaxin-3 (CCL26), Fas,(TNFRSF6/Apo-1), FGF-4, FGF-6, FGF-7 (KGF), FGF-9, Flt-3 Ligand, Fractalkine (CX3CL1), GCP-2 (CXCL6), GCSF, GDNF, GITR (TNFRSF18), GITR Ligand (TNFSF18), GM-CSF GRO alpha/beta/gamma, GRO alpha (CXCL1), HCC-4 (CCL16), HGF, I-309 (TCA-3/CCL1), CCAM-1 (CD54), ICAM-3 (CD50), IFN-gamma, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, IGF-1, IGF-1 R, IL-1 R4 (ST2), IL-1 R1, IL-10, IL-11, IL-12 p40, IL-12 p70, IL-13, IL-15, IL-16 IL-17A, IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-1 ra (IL-1 F3), IL-2, IL-2 R alpha, IL-3, IL-4, IL-5, IL-6, IL-6 R, IL-7, IL-8 (CXCL8), I-TAC (CXCL11), Leptin Light (TNFSF14), Lymphotactin (XCL1), MCP-1 (CCL2), MCP-2 (CCL8), MCP-3 (MARC/CCL7), MCP-4 (CCL13), M-CSF, MDC (CCL22), MIF, MIG (CXCL9), MIP-1 alpha (CCL3), MIP-1 beta (CCL4), MIP-1 delta (CCL15), MIP-3 alpha (CCL20), MIP-3 beta (CCL19), MSP alpha/beta, NAP-2 (PPBP/CXCL7), NT-3, NT-4, Oncostatin M, Osteoprotegerin (TNFRSF11B), PARC (CCL18), PDGF-BB, PLGF, RANTES (CCL5), SCF, SDF-1 alpha (CXCL12 alpha), gp130, TNF RII (TNFRSF1B), TNF RI (TNFRSF1A), TARC (CCL17), TECK (CCL25), TGF beta 3, TGF beta 1, Thrombopoietin (TPO), TIMP-1, TIMP-2, TNF alpha, TNF beta (TNFSF1B), TRAIL R3 (TNFRSF10C), TRAIL R4 (TNFRSF10D), uPAR, VEGF-A, VEGF-D.
    Learn more about membrane antibody arrays

  • Tested applications

    Suitable for: Multiplex Protein Detectionmore details

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 4 Membranes 1 x 8 Membranes 1 x 2 Membranes
    1,000X HRP-Streptavidin Buffer 1 x 50µl 2 x 50µl 1 x 50µl
    1X Blocking Buffer 2 x 25ml 4 x 25ml 1 x 25ml
    20X Wash Buffer I 1 x 20ml 2 x 20ml 1 x 10ml
    20X Wash Buffer II 1 x 20ml 2 x 20ml 1 x 10ml
    2X Cell Lysis Buffer 1 x 16ml 2 x 16ml 1 x 10ml
    8-Well Incubation Tray (with Lid) 1 unit 2 units 1 unit
    Biotinylated Antibody Cocktail (C6) 1 x 2 vials 1 x 4 vials 1 vial
    Biotinylated Antibody Cocktail (C7) 1 x 2 vials 1 x 4 vials 1 vial
    Detection Buffer C 1 x 2.5ml 2 x 2.5ml 1 x 1.5ml
    Detection Buffer D 1 x 2.5ml 2 x 2.5ml 1 x 1.5ml
    Human Cytokine Antibody Array Membranes (C6) 1 x 4 units 1 x 8 units 1 x 2 units
    Human Cytokine Antibody Array Membranes (C7) 1 x 4 units 1 x 8 units 1 x 2 units
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab193656 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Multiplex Protein Detection Use at an assay dependent concentration.

Images

  • Typical images obtained with Abcam Human Cytokine Antibody Array - Membrane C7 (120 Targets) ab193656. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at room temperature for 1 minute.

Protocols

References

This product has been referenced in:

  • Boeckmans J  et al. Elafibranor restricts lipogenic and inflammatory responses in a human skin stem cell-derived model of NASH. Pharmacol Res 144:377-389 (2019). Read more (PubMed: 31028903) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Questions for ab193656.
Please use the link above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up