Human Cytokine Antibody Array - Membrane (274 Targets) (ab198496)
Overview
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Product name
Human Cytokine Antibody Array - Membrane (274 Targets)
See all Cytokine Antibody Array antibody arrays -
Sample type
Serum, Plasma, Other biological fluids, Cell Lysate, Cell culture media, Tissue Lysate -
Assay type
Semi-quantitative -
Species reactivity
Reacts with: Human -
Product overview
ab198496 can be used for the simultaneous detection of 274 Human Cytokine proteins in serum, plasma, cell culture media and other liquid samples types. It comes as 1 kit including 5 separate membranes labelled C6, C7, C8, C9 and C10 (see below for images showing the array layout on each membrane).
Target names: 4-1BB (TNFRSF9/CD137), ACE-2, Adiponectin (ACRP30), Activin A, Adipsin (Complement Factor D), AgRP, ALCAM (CD166), Alpha-fetoprotein, Amphiregulin, Angiogenin, Angiopoietin-1, Angiopoietin-2, Angiostatin, ANGPTL4, Axl, CD80 (B7-1), Beta-2 Microglobulin, BCAM, BCMA (TNFRSF17), BDNF, beta IG-H3, bFGF, BLC (CXCL13), BMP-4, BMP-5, BMP-6, BMP-7, beta-NGF, Betacellulin (BTC) , CA125, CA15-3, CA19-9, CA9, Cardiotrophin-1 (CT-1), Cathepsin S, HCC-1 (CCL14), 6Ckine (CCL21) , CCL28 (MEC), CD14, CD23, CD30 (TNFRSF8), CD40 (TNFRSF5), CD40 Ligand (TNFSF5), CEA, CEACAM-1, CK beta 8-1 (CCL23), CNTF, Cripto-1, CRP (C-Reactive Protein), CTACK (CCL27), CXCL16, DAN, Decorin, DKK-1, Dkk-3, Dkk-4, CD26 (DPPIV), DR6 (TNFRSF21), Dtk, E-Cadherin, EDA-A2, EGF, EGFR, EG-VEGF (PK1), ENA-78 (CXCL5), Endoglin (CD105), Eotaxin-1 (CCL11), Eotaxin-2 (MPIF-2/CCL24), Eotaxin-3 (CCL26), TROP1 (EpCAM), ErbB2, ErbB3, Erythropoietin R, E-Selectin, Fas (TNFRSF6/Apo-1), Fas Ligand (TNFSF6), Fc gamma RIIB/C (CD32B/C), Ferritin, FGF-4, FGF-6, FGF-7 (KGF), FGF-9, Flt-3 Ligand, FLRG, Follistatin, Fractalkine (CX3CL1), FSH, Furin, Galectin-7, GCP-2 (CXCL6), GCSF, GDF-15, GDNF, GITR (TNFRSF18), GITR Ligand (TNFSF18), GM-CSF, GRO alpha/beta/gamma, GRO alpha (CXCL1), Growth Hormone, HB-EGF, HCC-4 (CCL16), hCG intact, HGF, HVEM (TNFRSF14), I-309 (TCA-3/CCL1), ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50) , IFN-gamma, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, IGF-1, IGF-1 R, IGF-2, IL-1 R2, IL-1 R4 (ST2), IL-1 R1, IL-10, IL-10 R alpha, IL-10 R beta, IL-11, IL-12 p40, IL-12 p70, IL-13, IL-13 R alpha 2, IL-13 R1, IL-15, IL-16, IL-17A, IL-17B, IL-17C, IL-17F, IL-17 RA, IL-18 BP alpha, IL-18 R beta (AcPL), IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-1 ra (IL-1 F3), IL-2, IL-2 R beta (CD122), IL-2 R gamma (Common gamma Chain), IL-2 R alpha, IL-21 R, IL-22, IL-28A (IFN-lambda 2), IL-29 (IFN-lambda 1), IL-3, IL-31, IL-4, IL-5, IL-5 R alpha, IL-6, IL-6 R, IL-7, IL-8 (CXCL8), IL-9, Insulin, IP-10 (CXCL10), I-TAC (CXCL11), LAP/TGF beta 1, Leptin, Leptin R, LIF, Light (TNFSF14), LIMPII, L-Selectin (CD62L), Luteinizing hormone, Lymphotactin (XCL1), LYVE-1, Marapsin, MCP-1 (CCL2), MCP-2 (CCL8), MCP-3 (MARC/CCL7), MCP-4 (CCL13), M-CSF, M-CSF R, MDC (CCL22), MICA, MICB, MIF, MIG (CXCL9), MIP-1 alpha (CCL3), MIP-1 beta (CCL4), MIP-1 delta (CCL15), MIP-3 alpha (CCL20), MIP-3 beta (CCL19), MMP-1, MMP-10, MMP-13, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MPIF-1 (CCL23), MSP alpha/beta, NAP-2 (PPBP/CXCL7), NCAM-1 (CD56), NGFR (TNFRSF16), Nidogen-1, NrCAM, NRG1-beta 1 (HRG1-beta 1), NT-3, NT-4, Oncostatin M, Osteopontin (SPP1), Osteoprotegerin (TNFRSF11B), PAI-1, PARC (CCL18), PDGF-AA, PDGF R alpha, PDGF R beta, PDGF-AB, PDGF-BB, PECAM-1 (CD31), PLGF, Platelet factor 4 (CXCL4), Procalcitonin, Prolactin, PSA-free, PSA-total, RAGE, RANK (TNFRSF11A), RANTES (CCL5), Resistin, S100 B, SAA (Serum Amyloid A), SCF, SCF R (CD117/c-kit), SDF-1 alpha (CXCL12 alpha), SDF-1 beta (CXCL12 beta), gp130, Sonic Hedgehog N-Terminal (Shh-N), Siglec-5 (CD170), Siglec-9, TNF RII (TNFRSF1B), TNF RI (TNFRSF1A), TACE, TARC (CCL17), TECK (CCL25), TGF beta 2, TGF alpha, TGF beta 3, TGF beta 1, Thrombopoietin (TPO), Thyroglobulin, Tie-1, Tie-2, TIM-1 (KIM-1), TIMP-1, TIMP-2, TIMP-4, TNF alpha, TNF beta (TNFSF1B), TRAIL R2 (TNFRSF10B/DR5), TRAIL R3 (TNFRSF10C), TRAIL R4 (TNFRSF10D), Trappin-2, TREM-1, TSH, TSLP, Ubiquitin+1, uPAR, VCAM-1 (CD106), VE-Cadherin (CDH5), VEGF-A, VEGFR2, VEGFR3, VEGF-C, VEGF-D, XEDAR.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Tested applications
Suitable for: Multiplex Protein Detectionmore details
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 2 Membranes 1 x 4 Membranes 1,000X HRP-Conjugated Streptavidin 2 x 50µl 3 x 50µl 1X Blocking Buffer 2 x 25ml 6 x 25ml 20X Wash Buffer I 2 x 20ml 3 x 20ml 20X Wash Buffer II 2 x 20ml 3 x 20ml 2X Cell Lysis Buffer 2 x 16ml 3 x 16ml 8-Well Incubation Tray (with Lid) 2 units 3 units Biotinylated Antibody Cocktail (C10) 1 vial 2 vials Biotinylated Antibody Cocktail (C6) 1 vial 2 vials Biotinylated Antibody Cocktail (C7) 1 vial 2 vials Biotinylated Antibody Cocktail (C8) 1 vial 2 vials Biotinylated Antibody Cocktail (C9) 1 vial 2 vials Detection Buffer C 2 x 2.5ml 3 x 2.5ml Detection Buffer D 2 x 2.5ml 3 x 2.5ml Human Cytokine Antibody Array Membranes (C10) 1 x 2 units 1 x 4 units Human Cytokine Antibody Array Membranes (C6) 1 x 2 units 1 x 4 units Human Cytokine Antibody Array Membranes (C7) 1 x 2 units 1 x 4 units Human Cytokine Antibody Array Membranes (C8) 1 x 2 units 1 x 4 units Human Cytokine Antibody Array Membranes (C9) 1 x 2 units 1 x 4 units -
Research areas
Applications
Our Abpromise guarantee covers the use of ab198496 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Multiplex Protein Detection | Use at an assay dependent concentration. |
Images
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Typical images obtained with Abcam Human Cytokine Antibody Array - Membrane (274 Targets) ab198496
Typical images obtained with Abcam Human Cytokine Antibody Array - Membrane (274 Targets) ab198496 Antibody Array. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed with film at room temperature for 1 minute.
References (0)
ab198496 has not yet been referenced specifically in any publications.
