Human Cytokine Antibody Array - Membrane (274 Targets) (ab198496)

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Overview

  • Product name

    Human Cytokine Antibody Array - Membrane (274 Targets)
    See all Cytokine Antibody Array antibody arrays

  • Sample type

    Serum, Plasma, Other biological fluids, Cell Lysate, Cell culture media, Tissue Lysate

  • Assay type

    Semi-quantitative

  • Species reactivity

    Reacts with: Human

  • Product overview

    ab198496 can be used for the simultaneous detection of 274 Human Cytokine proteins in serum, plasma, cell culture media and other liquid samples types. It comes as 1 kit including 5 separate membranes labelled C6, C7, C8, C9 and C10 (see below for images showing the array layout on each membrane). 


    Target names: 4-1BB (TNFRSF9/CD137), ACE-2, Adiponectin (ACRP30), Activin A, Adipsin (Complement Factor D), AgRP, ALCAM (CD166), Alpha-fetoprotein, Amphiregulin, Angiogenin, Angiopoietin-1, Angiopoietin-2, Angiostatin, ANGPTL4, Axl, CD80 (B7-1), Beta-2 Microglobulin, BCAM, BCMA (TNFRSF17), BDNF, beta IG-H3, bFGF, BLC (CXCL13), BMP-4, BMP-5, BMP-6, BMP-7, beta-NGF, Betacellulin (BTC) , CA125, CA15-3, CA19-9, CA9, Cardiotrophin-1 (CT-1), Cathepsin S, HCC-1 (CCL14), 6Ckine (CCL21) , CCL28 (MEC), CD14, CD23, CD30 (TNFRSF8), CD40 (TNFRSF5), CD40 Ligand (TNFSF5), CEA, CEACAM-1, CK beta 8-1 (CCL23), CNTF, Cripto-1, CRP (C-Reactive Protein), CTACK (CCL27), CXCL16, DAN, Decorin, DKK-1, Dkk-3, Dkk-4, CD26 (DPPIV), DR6 (TNFRSF21), Dtk, E-Cadherin, EDA-A2, EGF, EGFR, EG-VEGF (PK1), ENA-78 (CXCL5), Endoglin (CD105), Eotaxin-1 (CCL11), Eotaxin-2 (MPIF-2/CCL24), Eotaxin-3 (CCL26), TROP1 (EpCAM), ErbB2, ErbB3, Erythropoietin R, E-Selectin, Fas (TNFRSF6/Apo-1), Fas Ligand (TNFSF6), Fc gamma RIIB/C (CD32B/C), Ferritin, FGF-4, FGF-6, FGF-7 (KGF), FGF-9, Flt-3 Ligand, FLRG, Follistatin, Fractalkine (CX3CL1), FSH, Furin, Galectin-7, GCP-2 (CXCL6), GCSF, GDF-15, GDNF, GITR (TNFRSF18), GITR Ligand (TNFSF18), GM-CSF, GRO alpha/beta/gamma, GRO alpha (CXCL1), Growth Hormone, HB-EGF, HCC-4 (CCL16), hCG intact, HGF, HVEM (TNFRSF14), I-309 (TCA-3/CCL1), ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50) , IFN-gamma, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, IGF-1, IGF-1 R, IGF-2, IL-1 R2, IL-1 R4 (ST2), IL-1 R1, IL-10, IL-10 R alpha, IL-10 R beta, IL-11, IL-12 p40, IL-12 p70, IL-13, IL-13 R alpha 2, IL-13 R1, IL-15, IL-16, IL-17A, IL-17B, IL-17C, IL-17F, IL-17 RA, IL-18 BP alpha, IL-18 R beta (AcPL), IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-1 ra (IL-1 F3), IL-2, IL-2 R beta (CD122), IL-2 R gamma (Common gamma Chain), IL-2 R alpha, IL-21 R, IL-22, IL-28A (IFN-lambda 2), IL-29 (IFN-lambda 1), IL-3, IL-31, IL-4, IL-5, IL-5 R alpha, IL-6, IL-6 R, IL-7, IL-8 (CXCL8), IL-9, Insulin, IP-10 (CXCL10), I-TAC (CXCL11), LAP/TGF beta 1, Leptin, Leptin R, LIF, Light (TNFSF14), LIMPII, L-Selectin (CD62L), Luteinizing hormone, Lymphotactin (XCL1), LYVE-1, Marapsin, MCP-1 (CCL2), MCP-2 (CCL8), MCP-3 (MARC/CCL7), MCP-4 (CCL13), M-CSF, M-CSF R, MDC (CCL22), MICA, MICB, MIF, MIG (CXCL9), MIP-1 alpha (CCL3), MIP-1 beta (CCL4), MIP-1 delta (CCL15), MIP-3 alpha (CCL20), MIP-3 beta (CCL19), MMP-1, MMP-10, MMP-13, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MPIF-1 (CCL23), MSP alpha/beta, NAP-2 (PPBP/CXCL7), NCAM-1 (CD56), NGFR (TNFRSF16), Nidogen-1, NrCAM, NRG1-beta 1 (HRG1-beta 1), NT-3, NT-4, Oncostatin M, Osteopontin (SPP1), Osteoprotegerin (TNFRSF11B), PAI-1, PARC (CCL18), PDGF-AA, PDGF R alpha, PDGF R beta, PDGF-AB, PDGF-BB, PECAM-1 (CD31), PLGF, Platelet factor 4 (CXCL4), Procalcitonin, Prolactin, PSA-free, PSA-total, RAGE, RANK (TNFRSF11A), RANTES (CCL5), Resistin, S100 B, SAA (Serum Amyloid A), SCF, SCF R (CD117/c-kit), SDF-1 alpha (CXCL12 alpha), SDF-1 beta (CXCL12 beta), gp130, Sonic Hedgehog N-Terminal (Shh-N), Siglec-5 (CD170), Siglec-9, TNF RII (TNFRSF1B), TNF RI (TNFRSF1A), TACE, TARC (CCL17), TECK (CCL25), TGF beta 2, TGF alpha, TGF beta 3, TGF beta 1, Thrombopoietin (TPO), Thyroglobulin, Tie-1, Tie-2, TIM-1 (KIM-1), TIMP-1, TIMP-2, TIMP-4, TNF alpha, TNF beta (TNFSF1B), TRAIL R2 (TNFRSF10B/DR5), TRAIL R3 (TNFRSF10C), TRAIL R4 (TNFRSF10D), Trappin-2, TREM-1, TSH, TSLP, Ubiquitin+1, uPAR, VCAM-1 (CD106), VE-Cadherin (CDH5), VEGF-A, VEGFR2, VEGFR3, VEGF-C, VEGF-D, XEDAR. 


    Learn more about membrane antibody arrays

  • Tested applications

    Suitable for: Multiplex Protein Detectionmore details

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 2 Membranes 1 x 4 Membranes
    1,000X HRP-Conjugated Streptavidin 2 x 50µl 3 x 50µl
    1X Blocking Buffer 2 x 25ml 3 x 25ml
    20X Wash Buffer I 2 x 20ml 3 x 20ml
    20X Wash Buffer II 2 x 20ml 3 x 20ml
    2X Cell Lysis Buffer 2 x 16ml 3 x 16ml
    8-Well Incubation Tray (with Lid) 2 units 3 units
    Biotinylated Antibody Cocktail (C10) 1 vial 2 vials
    Biotinylated Antibody Cocktail (C6) 1 vial 2 vials
    Biotinylated Antibody Cocktail (C7) 1 vial 2 vials
    Biotinylated Antibody Cocktail (C8) 1 vial 2 vials
    Biotinylated Antibody Cocktail (C9) 1 vial 2 vials
    Detection Buffer C 2 x 2.5ml 3 x 2.5ml
    Detection Buffer D 2 x 2.5ml 3 x 2.5ml
    Human Cytokine Antibody Array Membranes (C10) 1 x 2 units 1 x 4 units
    Human Cytokine Antibody Array Membranes (C6) 1 x 2 units 1 x 4 units
    Human Cytokine Antibody Array Membranes (C7) 1 x 2 units 1 x 4 units
    Human Cytokine Antibody Array Membranes (C8) 1 x 2 units 1 x 4 units
    Human Cytokine Antibody Array Membranes (C9) 1 x 2 units 1 x 4 units

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab198496 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Multiplex Protein Detection Use at an assay dependent concentration.

Images

  • Typical images obtained with Abcam Human Cytokine Antibody Array - Membrane (274 Targets) ab198496
    Typical images obtained with Abcam Human Cytokine Antibody Array - Membrane (274 Targets) ab198496

    Typical images obtained with Abcam Human Cytokine Antibody Array - Membrane (274 Targets) ab198496 Antibody Array. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed with film at room temperature for 1 minute.

Protocols

References

ab198496 has not yet been referenced specifically in any publications.

Publishing research using ab198496? Please let us know so that we can cite the reference in this datasheet.

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