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    human-cytokine-antibody-array-membrane-274-targets-ab198496.pdf

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Immunology Innate Immunity Cytokines Interleukins
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Human Cytokine Antibody Array - Membrane (274 Targets) (ab198496)

  • Datasheet
  • SDS
  • Protocol Booklet
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Typical images obtained with Abcam Human Cytokine Antibody Array - Membrane (274 Targets) ab198496

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    Overview

    • Product name

      Human Cytokine Antibody Array - Membrane (274 Targets)
      See all Cytokine Antibody Array antibody arrays
    • Sample type

      Serum, Plasma, Other biological fluids, Cell Lysate, Cell culture media, Tissue Lysate
    • Assay type

      Semi-quantitative
    • Species reactivity

      Reacts with: Human
    • Product overview

      ab198496 can be used for the simultaneous detection of 274 Human Cytokine proteins in serum, plasma, cell culture media and other liquid samples types. It comes as 1 kit including 5 separate membranes labelled C6, C7, C8, C9 and C10 (see below for images showing the array layout on each membrane). 


      Target names: 4-1BB (TNFRSF9/CD137), ACE-2, Adiponectin (ACRP30), Activin A, Adipsin (Complement Factor D), AgRP, ALCAM (CD166), Alpha-fetoprotein, Amphiregulin, Angiogenin, Angiopoietin-1, Angiopoietin-2, Angiostatin, ANGPTL4, Axl, CD80 (B7-1), Beta-2 Microglobulin, BCAM, BCMA (TNFRSF17), BDNF, beta IG-H3, bFGF, BLC (CXCL13), BMP-4, BMP-5, BMP-6, BMP-7, beta-NGF, Betacellulin (BTC) , CA125, CA15-3, CA19-9, CA9, Cardiotrophin-1 (CT-1), Cathepsin S, HCC-1 (CCL14), 6Ckine (CCL21) , CCL28 (MEC), CD14, CD23, CD30 (TNFRSF8), CD40 (TNFRSF5), CD40 Ligand (TNFSF5), CEA, CEACAM-1, CK beta 8-1 (CCL23), CNTF, Cripto-1, CRP (C-Reactive Protein), CTACK (CCL27), CXCL16, DAN, Decorin, DKK-1, Dkk-3, Dkk-4, CD26 (DPPIV), DR6 (TNFRSF21), Dtk, E-Cadherin, EDA-A2, EGF, EGFR, EG-VEGF (PK1), ENA-78 (CXCL5), Endoglin (CD105), Eotaxin-1 (CCL11), Eotaxin-2 (MPIF-2/CCL24), Eotaxin-3 (CCL26), TROP1 (EpCAM), ErbB2, ErbB3, Erythropoietin R, E-Selectin, Fas (TNFRSF6/Apo-1), Fas Ligand (TNFSF6), Fc gamma RIIB/C (CD32B/C), Ferritin, FGF-4, FGF-6, FGF-7 (KGF), FGF-9, Flt-3 Ligand, FLRG, Follistatin, Fractalkine (CX3CL1), FSH, Furin, Galectin-7, GCP-2 (CXCL6), GCSF, GDF-15, GDNF, GITR (TNFRSF18), GITR Ligand (TNFSF18), GM-CSF, GRO alpha/beta/gamma, GRO alpha (CXCL1), Growth Hormone, HB-EGF, HCC-4 (CCL16), hCG intact, HGF, HVEM (TNFRSF14), I-309 (TCA-3/CCL1), ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50) , IFN-gamma, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, IGF-1, IGF-1 R, IGF-2, IL-1 R2, IL-1 R4 (ST2), IL-1 R1, IL-10, IL-10 R alpha, IL-10 R beta, IL-11, IL-12 p40, IL-12 p70, IL-13, IL-13 R alpha 2, IL-13 R1, IL-15, IL-16, IL-17A, IL-17B, IL-17C, IL-17F, IL-17 RA, IL-18 BP alpha, IL-18 R beta (AcPL), IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-1 ra (IL-1 F3), IL-2, IL-2 R beta (CD122), IL-2 R gamma (Common gamma Chain), IL-2 R alpha, IL-21 R, IL-22, IL-28A (IFN-lambda 2), IL-29 (IFN-lambda 1), IL-3, IL-31, IL-4, IL-5, IL-5 R alpha, IL-6, IL-6 R, IL-7, IL-8 (CXCL8), IL-9, Insulin, IP-10 (CXCL10), I-TAC (CXCL11), LAP/TGF beta 1, Leptin, Leptin R, LIF, Light (TNFSF14), LIMPII, L-Selectin (CD62L), Luteinizing hormone, Lymphotactin (XCL1), LYVE-1, Marapsin, MCP-1 (CCL2), MCP-2 (CCL8), MCP-3 (MARC/CCL7), MCP-4 (CCL13), M-CSF, M-CSF R, MDC (CCL22), MICA, MICB, MIF, MIG (CXCL9), MIP-1 alpha (CCL3), MIP-1 beta (CCL4), MIP-1 delta (CCL15), MIP-3 alpha (CCL20), MIP-3 beta (CCL19), MMP-1, MMP-10, MMP-13, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MPIF-1 (CCL23), MSP alpha/beta, NAP-2 (PPBP/CXCL7), NCAM-1 (CD56), NGFR (TNFRSF16), Nidogen-1, NrCAM, NRG1-beta 1 (HRG1-beta 1), NT-3, NT-4, Oncostatin M, Osteopontin (SPP1), Osteoprotegerin (TNFRSF11B), PAI-1, PARC (CCL18), PDGF-AA, PDGF R alpha, PDGF R beta, PDGF-AB, PDGF-BB, PECAM-1 (CD31), PLGF, Platelet factor 4 (CXCL4), Procalcitonin, Prolactin, PSA-free, PSA-total, RAGE, RANK (TNFRSF11A), RANTES (CCL5), Resistin, S100 B, SAA (Serum Amyloid A), SCF, SCF R (CD117/c-kit), SDF-1 alpha (CXCL12 alpha), SDF-1 beta (CXCL12 beta), gp130, Sonic Hedgehog N-Terminal (Shh-N), Siglec-5 (CD170), Siglec-9, TNF RII (TNFRSF1B), TNF RI (TNFRSF1A), TACE, TARC (CCL17), TECK (CCL25), TGF beta 2, TGF alpha, TGF beta 3, TGF beta 1, Thrombopoietin (TPO), Thyroglobulin, Tie-1, Tie-2, TIM-1 (KIM-1), TIMP-1, TIMP-2, TIMP-4, TNF alpha, TNF beta (TNFSF1B), TRAIL R2 (TNFRSF10B/DR5), TRAIL R3 (TNFRSF10C), TRAIL R4 (TNFRSF10D), Trappin-2, TREM-1, TSH, TSLP, Ubiquitin+1, uPAR, VCAM-1 (CD106), VE-Cadherin (CDH5), VEGF-A, VEGFR2, VEGFR3, VEGF-C, VEGF-D, XEDAR. 


      Learn more about membrane antibody arrays

    • Notes

      Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
      It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

    • Tested applications

      Suitable for: Multiplex Protein Detectionmore details

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components 1 x 2 Membranes 1 x 4 Membranes
      1,000X HRP-Conjugated Streptavidin 2 x 50µl 3 x 50µl
      1X Blocking Buffer 2 x 25ml 6 x 25ml
      20X Wash Buffer I 2 x 20ml 3 x 20ml
      20X Wash Buffer II 2 x 20ml 3 x 20ml
      2X Cell Lysis Buffer 2 x 16ml 3 x 16ml
      8-Well Incubation Tray (with Lid) 2 units 3 units
      Biotinylated Antibody Cocktail (C10) 1 vial 2 vials
      Biotinylated Antibody Cocktail (C6) 1 vial 2 vials
      Biotinylated Antibody Cocktail (C7) 1 vial 2 vials
      Biotinylated Antibody Cocktail (C8) 1 vial 2 vials
      Biotinylated Antibody Cocktail (C9) 1 vial 2 vials
      Detection Buffer C 2 x 2.5ml 3 x 2.5ml
      Detection Buffer D 2 x 2.5ml 3 x 2.5ml
      Human Cytokine Antibody Array Membranes (C10) 1 x 2 units 1 x 4 units
      Human Cytokine Antibody Array Membranes (C6) 1 x 2 units 1 x 4 units
      Human Cytokine Antibody Array Membranes (C7) 1 x 2 units 1 x 4 units
      Human Cytokine Antibody Array Membranes (C8) 1 x 2 units 1 x 4 units
      Human Cytokine Antibody Array Membranes (C9) 1 x 2 units 1 x 4 units
    • Research areas

      • Immunology
      • Innate Immunity
      • Cytokines
      • Interleukins
      • Immunology
      • Innate Immunity
      • Cytokines
      • Interferons
      • Antibody Arrays
      • Membrane antibody arrays
      • Cytokine antibody arrays

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab198496 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Multiplex Protein Detection
    Use at an assay dependent concentration.
    Notes
    Multiplex Protein Detection
    Use at an assay dependent concentration.

    Images

    • Typical images obtained with Abcam Human Cytokine Antibody Array - Membrane (274 Targets) ab198496
      Typical images obtained with Abcam Human Cytokine Antibody Array - Membrane (274 Targets) ab198496

      Typical images obtained with Abcam Human Cytokine Antibody Array - Membrane (274 Targets) ab198496 Antibody Array. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed with film at room temperature for 1 minute.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab198496? Please let us know so that we can cite the reference in this datasheet.

    ab198496 has not yet been referenced specifically in any publications.

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