Human Cytokine Antibody Array - Membrane (54 targets) (ab169816)

Overview

  • Product name
    Human Cytokine Antibody Array - Membrane (54 targets)
    See all Cytokine Antibody Array antibody arrays
  • Sample type
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture media
  • Assay type
    Semi-quantitative
  • Species reactivity
    Reacts with: Human
  • Product overview

    ab169816 is for the simultaneous detection of 54 Human Cytokines in 4 or 8 samples. Suitable for all liquid sample types.


    Targets: Activin A, ALCAM, CD80, BMP-5, BMP-7, Cardiotrophin-1, CD14, CXCL16/ DR6 , TNFRSF21, Endoglin, ErbB3, E-Selectin, Fas Ligand, ICAM-2, IGF-2, IL-1 RII, IL-10 R beta, IL-13 R alpha 2, IL-18 BP alpha, IL-18 R beta, MMP-3, IL-2 R beta, IL-2 R gamma, IL-21R, IL-5 R alpha, IL-9, IP-10, TGF beta 1, Leptin R, LIF, L-Selectin, M-CSF R, MMP-1, MMP-13, MMP-9, MPIF-1, NGF R, PDGF-AA, PDGF-AB, PDGF R alpha, PDGF R beta, PECAM-1, Prolactin, SCF R, SDF-1 beta, Siglec-5, TGF alpha, TGF beta 2, Tie-1, Tie-2, TIMP-4, VE-Cadherin, VEGFR2, VEGFR3


    Learn more about membrane antibody arrays

  • Notes

    If you are interested in this cytokine array, array ab134004 may also be of interest. A list of human antibody membrane arrays is available here.

  • Tested applications
    Suitable for: Multiplex Protein Detectionmore details

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 4 Membranes 1 x 8 Membranes 1 x 2 Membranes
    1,000X HRP-Streptavidin Buffer 1 x 50µl 1 x 50µl 1 x 50µl
    1X Blocking Buffer 1 x 25ml 1 x 50ml 1 x 25ml
    20X Wash Buffer I 1 x 10ml 1 x 20ml 1 x 10ml
    20X Wash Buffer II 1 x 10ml 1 x 20ml 1 x 10ml
    2X Cell Lysis Buffer 1 x 10ml 1 x 20ml 1 x 10ml
    8-Well Incubation Tray (with Lid) 1 unit 1 unit 1 unit
    Biotinylated Antibody Cocktail (C8) 1 x 2 vials 1 x 4 vials 1 x 2 vials
    Human Cytokine Antibody Array Membranes (C8) 1 x 4 units 1 x 8 units 1 x 2 units
    Detection Buffer C 1 x 1.5ml 1 x 2.5ml 1 x 1.5ml
    Detection Buffer D 1 x 1.5ml 1 x 2.5ml 1 x 1.5ml
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab169816 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Multiplex Protein Detection Use at an assay dependent concentration.

Images

  • Typical images obtained with Abcam Human Cytokine Antibody Array. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at room temperature for 1 minute.

Protocols

References

ab169816 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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