Overview

  • Product name
    Human Cytokines FirePlex®-HT Panel 1
  • Detection method
    Fluorescent
  • Sample type
    Cell culture supernatant, Serum, Plasma, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type
    Multiplex
  • Species reactivity
    Reacts with: Human
  • Product overview

    Human Cytokines FirePlex®-HT Panel 1 (ab234897) uses FirePlex® particle technology to quantify IL-8, IFN gamma, IL-4, IL-1 beta, MCP1, TNF alpha, IL-10, IL-17A, IL-2, IL-6 from 6.25 µl of sample input in 384-well plate format.


    Assays are validated in serum, plasma, and cell culture supernatant with 175 samples in duplicate per 384-well plate.


    The two-step workflow and no-wash assay format limit hands-on time and are amenable to automation, thus making FirePlex-HT ideally suited for high-throughput screening studies. Assay readout is conducted on high-content imagers with data analysis using the FirePlex® Analysis Workbench software.


    Users should note that assay performance data supplied in the accompanying Product Datasheet is a representative dataset, and is not lot-specific. For lot-specific assay performance data, please refer to the product's certificate of analysis, which can be found here.


    FirePlex® is a registered trade mark in certain territories (including Europe and the United States) and is an unregistered trade mark elsewhere

  • Notes

    This product is currently only supported for customers in North America and Europe, if you are outside these areas please contact us.

    The following table provides the sensitivity, dynamic range, and CVs for Human Cytokines FirePlex®-HT Panel 1 *.

    Target 

    Sensitivity/LOD

    (pg/ml) 

    Dynamic Range

    (pg/ml) 

     Inter-well CV

    (Std curve)

     IL-8 5.72 13.7 - 3,333  7.4%
     IFN-gamma 72.48 123.4 - 10,000  11.5%
     IL-4 19.96 41.1 - 10,000 5.9%
     IL-1 beta 4.54 4.5 - 3,333 16.1%
     MCP1 2.43 4.5 - 3,333  13.3%
     TNF-alpha 18.03 41.1 - 10,000 4.7%
     IL-10 22.75 41.1-10,000 9.0%
     IL-17A 7.04 13.7-10,000 13.4%
     IL-2 13.51 13.7 - 10,000 5.2%
     IL-6 3.06 4.5 - 10,000 8.4%

    *representative dataset, not lot-specific. For lot-specific assay performance data, please refer to the product's certificate of analysis, which can be found here.

  • Platform
    Multiplex

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Protocols

References

ab234897 has not yet been referenced specifically in any publications.

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