Product nameHuman Anti-Cytomegalovirus IgG ELISA Kit (CMV)
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s anti-Cytomegalovirus (CMV) IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Cytomegalovirus in Human serum.
A 96-well plate has been precoated with Cytomegalovirus antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Cytomegalovirus antigens. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Cytomegalovirus IgG sample captured in plate.
Tested applicationsSuitable for: ELISAmore details
Storage instructionsStore at +4°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Washing Solution 1 x 50ml Cytomegalovirus (IgG) Coated Microplate (12 x 8 wells) 1 unit Cytomegalovirus anti-IgG HRP Conjugate 1 x 12ml Cytomegalovirus IgG Negative Control Blue cap 1 x 100µl Cytomegalovirus IgG Positive Control Purple cap 1 x 100µl Cytomegalovirus IgG Standard 0 - 0 U/mL Natural cap 1 x 100µl Cytomegalovirus IgG Standard 1 - 1.2 U/mL Yellow cap 1 x 100µl Cytomegalovirus IgG Standard 2 - 6 U/mL Red cap 1 x 100µl Cytomegalovirus IgG Standard 3 - 18 U/mL Green cap 1 x 100µl IgG Sample Diluent 1 x 22ml Stop Solution 1 x 11ml TMB Reagent 1 x 11ml
Our Abpromise guarantee covers the use of ab108639 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent dilution.|
This product has been referenced in:
- Lustig A et al. Telomere Shortening, Inflammatory Cytokines, and Anti-Cytomegalovirus Antibody Follow Distinct Age-Associated Trajectories in Humans. Front Immunol 8:1027 (2017). Read more (PubMed: 28970831) »
- Lin Y et al. Changes in blood lymphocyte numbers with age in vivo and their association with the levels of cytokines/cytokine receptors. Immun Ageing 13:24 (2016). ELISA ; Human . Read more (PubMed: 27547234) »