Human Anti-Cytomegalovirus IgG ELISA Kit (CMV) (ab108724)

Reviews (1)Q&A (5)References (4)

Overview

  • Product name

    Human Anti-Cytomegalovirus IgG ELISA Kit (CMV)

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Pos.Serum 12 1.9%
    Inter-assay
    Sample n Mean SD CV%
    Pos.Serum 4 6.2%

  • Sample type

    Serum, Heparin Plasma, Citrate Plasma

  • Assay type

    Indirect

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Abcam’s anti-Cytomegalovirus (CMV) IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the qualitative measurement of IgG class antibodies against Cytomegalovirus in Human serum and plasma.

     

    A 96-well plate has been precoated with Cytomegalovirus antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Cytomegalovirus-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Cytomegalovirus IgG sample captured in plate.

  • Tested applications

    Suitable for: Indirect ELISAmore details

  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108724 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA Use at an assay dependent concentration.

Protocols

References

This product has been referenced in:

  • Salovin A  et al. Anti-NMDA receptor encephalitis and nonencephalitic HSV-1 infection. Neurol Neuroimmunol Neuroinflamm 5:e458 (2018). Read more (PubMed: 29629396) »
  • Przemska-Kosicka A  et al. Age-Related Changes in the Natural Killer Cell Response to Seasonal Influenza Vaccination Are Not Influenced by a Synbiotic: a Randomised Controlled Trial. Front Immunol 9:591 (2018). Read more (PubMed: 29662493) »
See all 4 Publications for this product

Publishing research using ab108724? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-6 of 6 Abreviews or Q&A

Quantification of IgG anti-CMV ELISA in frozen plasma samples with ab108724 Anti-cytomegalovirus (CMV) IgG human ELISA kit

 Excellent Excellent 5/5 (Ease of Use)
Abreviews
abreview image
Quantification of human IgG anti-CMV in EDTA plasma samples (freshly frozen and stored at -80C) was tested using the ab108724 ELISA kit. The information provided with the kit stated that only serum samples and citrate plasma samples had been previously tested. We wanted to test whether the kit allowed accurate quantification of human IgG anti-CMV in EDTA plasma.
Plasma samples were diluted 1:100 with the sample diluent provided with the kit (as per the manufacturer’s instructions) and assayed in duplicate. Duplicate wells for positive control, cut-off and negative control as well as blank were included as per the protocol provided. All steps followed the instructions provided with the ELISA kit.
Absorbance (optical density, OD) was determined with a plate reader set at 450 nm with a reference wavelength of 650 nm. Absorbance of blank wells was averaged and subtracted from all other wells. Absorbance values were converted into standard units as instructed by the manufacturer (cut-off = 10 units positive > 11 units negative < 9 units grey zone = 9-11 units).
The kit distinguished clearly between IgG anti-CMV positive and negative samples and allowed quantification of these antibodies in EDTA plasma samples (see Figure).
The protocol provided with the kit was clear and easy to follow and contained all the information necessary to perform the assay.

Abcam user community

Verified customer

Submitted Sep 04 2019

Question

Do you sell just the antigen for the below and if so at what cost?

Adenovirus IgG Human ELISA Kit (ab108705)
Measles Virus IgG Human ELISA Kit (ab108750)
Cytomegalovirus IgG (CMV IgG) Human ELISA Kit (ab108724)
Epstein-Barr Virus (EBNA) IgG Human ELISA Kit (ab108731)

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Answer

Thank you for your email. I do apologize for the delay in responding to your email.

We unfortunately do not sell the antigens for these kits seperately.

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Question

We would be interested in obtaining a quote for the ToRCH range of assays. In particular:


Toxoplasma IgG

Toxoplasma IgM

Rubella IgG

Rubella IgM

CMV IgG

CMV IgM

HSV-1 IgG

HSV-2 IgG

HSV-1 & 2 IgM


We look forward to hearing from you soon.

Kind regards,

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Answer

Thank you for your email.

The product I have identified for you are as follows;

ab108776
ab108778

ab108767
we do not have anti rubella IgM kit

ab108724
ab108725

ab108737
ab108738

ab108739
ab108740

ab108742

I have contacted my colleague who will give you a bulk quote for this kit. Please let me know if you do not hear from us within 24 hours of this email.

I hope this information will be helpful. Should you require more information please do not hesitate to contact me.

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Question

Interested in finding out which antigen is coated on ab108724. Is working on mouse CMV and wants to know what antigens human kits employ

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Answer

Thank you for your inquiry.



I am happy to confirm that the plates of ab108724 is coated with CMV Strain AD-169 Antigens.

I hope this information is helpful. Please do not hesitate to contact em again with any further questions.

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Question

I have a few questions about ab108724? 1. What is the detection limit? 2. What are "cut off" controls? 3. How are controls to be prepared?

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Answer

A1: The CMV ELISA, ab108724, is qualitative and therefore does not have a known detection limit. The assay sensitivity is defined as the probability of the assay scoring positive in the presence of specific analyte. The assay sensitivity for this kit is 98%. A2: The cut-off control is used to determine the optical density threshold at which samples are considered positive or negative. Please see the product insert for details on this calculation. A3: The controls in this kit come in liquid form ready to use.

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Question

 I have some questions to ask regarding CMV IgG Human ELISA Kit # ab108724. It would be really appreciated if you could provide me answers to the following questions. 1.       What is the limit of detection and specificity of the assay? 2.       Please provide some guidance on spiking experiments? 3.       Any recommendation for heat-denaturing of serum to mitigate albumin interference? 4.       Any known non-specific binding concerns that we can address to this assay?  

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Answer

Thank you for your patience. The laboratory that developed the ELISA has sent the following information. If it does not completely answer your questions, please contact me. The CMV IgG ELISA is a qualitative test. Therefore we do not have data about the detection limit or spiking. Heat inactivation of samples is not recommended since it influences the test results in a negative way. Cross reactivity with antibodies against HSV1-2, Measles, Mumps, or VZV have not been observed. The diagnostics specificity is defined as the probability of the assay scoring negative in the absence of specific analyte. For this, it is 97.5%. The diagnostic senstivity is defined as the probability of the assay scoring positive in the presence of specific analyte. The sensitivity of this assay is 98%. Interferences with hemolytic, lipemic, or icteric sera are not ovserved up to a concentration of 10 mg/ml hemoglobin, 5 mg/ml triglycerides, and 0.2 mg/ml bilirubin.  

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