Human Anti-Cytomegalovirus IgM ELISA Kit (CMV) (ab108725)

Overview

  • Product name

    Human Anti-Cytomegalovirus IgM ELISA Kit (CMV)
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Pos.Serum 11 5.6%
    Inter-assay
    Sample n Mean SD CV%
    Pos.Serum 25 7.9%
  • Sample type

    Serum, Heparin Plasma, Citrate Plasma
  • Assay type

    Indirect
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s anti-Cytomegalovirus (CMV) IgM Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgM class antibodies against Cytomegalovirus in Human serum and plasma.

     

    A 96-well plate has been precoated with Cytomegalovirus antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgM conjugate is added to the wells, which binds to the immobilized Cytomegalovirus-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Cytomegalovirus IgM sample captured in plate.

  • Tested applications

    Suitable for: Indirect ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108725 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA Use at an assay dependent concentration.

Protocols

References

ab108725 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Abtrial REFERENCES: 190807-002151A (ab108725)

Quantification of human IgM anti-CMV in EDTA plasma samples (freshly frozen and stored at -80C) was tested using the ab108724 ELISA kit. The information provided with the kit stated that only serum samples and citrate plasma samples had been previously tested. We wanted to test whether the kit allowed accurate quantification of human IgM anti-CMV in EDTA plasma.

Plasma samples were diluted 1:100 with the sample diluent provided with the kit (as per the manufacturer’s instructions) and assayed in duplicate. Duplicate wells for positive control, cut-off and negative control as well as blank were included as per the protocol provided. All steps followed the instructions provided with the ELISA kit.

Absorbance (optical density, OD) was determined with a plate reader set at 450 nm with a reference wavelength of 650 nm. Absorbance of blank wells was averaged and subtracted from all other wells. Absorbance values were converted into standard units as instructed by the manufacturer (cut-off = 10 units positive > 11 units negative < 9 units grey zone = 9-11 units).

The kit distinguished clearly between IgM anti-CMV positive and negative samples and allowed quantification of these antibodies in EDTA plasma samples (see Figure).

The protocol provided with the kit was clear and easy to follow and contained all the information necessary to perform the assay.

Abcam user community

Verified customer

Submitted Sep 06 2019

Answer

Thank you for your email.

The product I have identified for you are as follows;

ab108776
ab108778

ab108767
we do not have anti rubella IgM kit

ab108724
ab108725

ab108737
ab108738

ab108739
ab108740

ab108742

I have contacted my colleague who will give you a bulk quote for this kit. Please let me know if you do not hear from us within 24 hours of this email.

I hope this information will be helpful. Should you require more information please do not hesitate to contact me.

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