Key features and details
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
Product nameHuman Anti-Cytomegalovirus IgM ELISA Kit (CMV)
Intra-assay Sample n Mean SD CV% Pos.Serum 11 5.6% Inter-assay Sample n Mean SD CV% Pos.Serum 25 7.9%
Sample typeSerum, Hep Plasma, Cit plasma
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s anti-Cytomegalovirus (CMV) IgM Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgM class antibodies against Cytomegalovirus in Human serum and plasma.
A 96-well plate has been precoated with Cytomegalovirus antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgM conjugate is added to the wells, which binds to the immobilized Cytomegalovirus-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Cytomegalovirus IgM sample captured in plate.
Storage instructionsStore at +4°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Cover foil 1 unit Cytomegalovirus antigens Coated Microplate (12 x 8 wells) 1 unit Cytomegalovirus anti-IgM HRP Conjugate Black cap 1 x 20ml Cytomegalovirus IgM Cut-off Control Green cap 1 x 3ml Cytomegalovirus IgM Negative Control Blue cap 1 x 2ml Cytomegalovirus IgM Positive Control Red cap 1 x 2ml IgM Sample Diluent White cap 1 x 100ml Stop Solution red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml
ab108725 has not yet been referenced specifically in any publications.