Overview

  • Product name
    Human D-Dimer ELISA Kit
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 5 3%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 5%
  • Sample type
    Cell culture supernatant, Urine, Serum, EDTA Plasma, Citrate Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    71 pg/ml
  • Range
    0.475 ng/ml - 30 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Urine 99 98% - 101%
    Serum 105 104% - 107%
    EDTA Plasma 104 100% - 107%
    Citrate Plasma 105 102% - 109%

  • Assay time
    1h 30m
  • Assay duration
    One step assay
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat, Rabbit, Goat, Guinea pig, Hamster, Cow, Dog, Pig
  • Product overview

    Abcam’s D-Dimer in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Fibrinogen D-Dimer protein in Human serum, plasma, cell culture supernatant and urine samples.


    NOTE: This kit will recognize D-Dimer and other Fibrin Degradation Products (FDPs).


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    D-dimer is a protein formed by the cross-linking of two D fragments of the fibrin protein. D-dimer is one of several fibrin degradation product (FDP) formed by the degradation of a blood clot by fibrinolysis. Its measurement is used to diagnose the blood disorder disseminated intravascular coagulation and in the diagnosis of thrombosis.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human D-Dimer Capture Antibody 1 x 600µl
    10X Human D-Dimer Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    ab221824 - Antibody Diluent 4BI 1 x 6ml
    Human D-Dimer Lyophilized Purified Protein 2 vials
    Plate Seals 1 unit
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml

Applications

Our Abpromise guarantee covers the use of ab196269 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • ELISA Protocol Summary
  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD, n = 3) are graphed.

  • Ten individual healthy donors were evaluated for the presence of D-Dimer in serum using this assay.

Protocols

References

This product has been referenced in:
  • Gao R  et al. The Value of Combining Plasma D-Dimer and Endothelin-1 Levels to Predict No-Reflow After Percutaneous Coronary Intervention of ST-Segment Elevation in Acute Myocardial Infarction Patients with a Type 2 Diabetes Mellitus History. Med Sci Monit 24:3549-3556 (2018). Read more (PubMed: 29806659) »
  • Grzanka R  et al. Interplay between acute phase response and coagulation/fibrinolysis in chronic spontaneous urticaria. Allergy Asthma Clin Immunol 14:27 (2018). Read more (PubMed: 30026764) »
See all 2 Publications for this product

Customer reviews and Q&As

Question
Answer

The antibodies in this kit were made using homogenized human fibrin clot, D-dimer or high molecular weight fibrin degradation products. The homology between human and monkey fibrinogen is high (91%) so the kit is likely to work in monkey.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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