Product nameHuman D-Dimer ELISA Kit
Intra-assay Sample n Mean SD CV% Overall 5 3% Inter-assay Sample n Mean SD CV% Overall 3 5%
Sample typeCell culture supernatant, Urine, Serum, EDTA Plasma, Citrate Plasma
Assay typeSandwich (quantitative)
Range0.475 ng/ml - 30 ng/ml
Sample specific recovery Sample type Average % Range Urine 99 98% - 101% Serum 105 104% - 107% EDTA Plasma 104 100% - 107% Citrate Plasma 105 102% - 109%
Assay time1h 30m
Assay durationOne step assay
Species reactivityReacts with: Human
Does not react with: Mouse, Rat, Rabbit, Goat, Guinea pig, Hamster, Cow, Dog, Pig
Abcam’s D-Dimer in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Fibrinogen D-Dimer protein in Human serum, plasma, cell culture supernatant and urine samples.
NOTE: This kit will recognize D-Dimer and other Fibrin Degradation Products (FDPs).
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
D-dimer is a protein formed by the cross-linking of two D fragments of the fibrin protein. D-dimer is one of several fibrin degradation product (FDP) formed by the degradation of a blood clot by fibrinolysis. Its measurement is used to diagnose the blood disorder disseminated intravascular coagulation and in the diagnosis of thrombosis.
Tested applicationsSuitable for: Sandwich ELISAmore details
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Human D-Dimer Capture Antibody 1 x 600µl 10X Human D-Dimer Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml ab221824 - Antibody Diluent 4BI 1 x 6ml Human D-Dimer Lyophilized Purified Protein 2 vials Plate Seals 1 unit SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Sample Diluent NS (ab193972) 1 x 50ml Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml
Our Abpromise guarantee covers the use of ab196269 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
This product has been referenced in:
- Gao R et al. The Value of Combining Plasma D-Dimer and Endothelin-1 Levels to Predict No-Reflow After Percutaneous Coronary Intervention of ST-Segment Elevation in Acute Myocardial Infarction Patients with a Type 2 Diabetes Mellitus History. Med Sci Monit 24:3549-3556 (2018). Read more (PubMed: 29806659) »
- Grzanka R et al. Interplay between acute phase response and coagulation/fibrinolysis in chronic spontaneous urticaria. Allergy Asthma Clin Immunol 14:27 (2018). Read more (PubMed: 30026764) »