Human D-Dimer ELISA Kit (ab260076)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 2.36 ng/ml
- Range: 4.69 ng/ml - 300 ng/ml
- Sample type: Cell culture media, Cit plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human D-Dimer ELISA Kit
See all D-Dimer kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 4.4% Inter-assay Sample n Mean SD CV% Serum 3 4.3% -
Sample type
Urine, Serum, Cell culture media, Hep Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
2.36 ng/ml -
Range
4.69 ng/ml - 300 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Urine 101 99% - 103% Serum 107 96% - 124% Tissue Culture Media 83 81% - 84% Hep Plasma 101 99% - 103% Cit plasma 98 95% - 105% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Cow -
Product overview
Human D-Dimer ELISA Kit (ab260076) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of D-Dimer protein in hep plasma, serum, urine, cell culture media, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human D-Dimer with 2.36 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Plasma collected with EDTA is not compatible with this kit.
ASSAY SPECIFICITY
This kit recognizes native human D-Dimer protein in serum, plasma (citrate), plasma (heparin), cell and tissue culture supernatant, and urine samples only.
Cell and tissue extract samples have not been tested with this kit.
CROSS REACTIVITY
Recombinant human fibrinogen, plasminogen, angiostatin, tPA, PAI1, and plasmin were prepared at 150 ng/mL, diluted serially, and assayed for cross reactivity. No cross-reactivity was observed.
INTERFERENCE
Recombinant human fibrinogen, plasminogen, angiostatin, tPA, PAI1, and plasmin were prepared at 150 ng/mL, diluted serially, and assayed for interference with human D-Dimer. No cross-reactivity was observed.
SPECIES REACTIVITY
This kit recognizes human D-Dimer protein.
Other species reactivity was determined by measuring 1: 200 mouse, rat, and bovine serum samples, interpolating the D-Dimer protein concentrations from the human standard curve, and expressing the interpolated concentrations as a percentage of the D-Dimer protein concentration in human serum assayed at the same dilution.
Reactivity < 3% was determined for the following species: Mouse, Rat, Cow
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Notes
D-dimer is a protein formed by the cross-linking of two D fragments of the fibrin protein. D-dimer is one of several fibrin degradation products (FDPs) formed by the degradation of a blood clot by fibrinolysis. Its measurement is used to diagnose the blood disorder disseminated intravascular coagulation and in the diagnosis of thrombosis
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human D-Dimer Capture Antibody 1 x 600µl 10X Human D-Dimer Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human D-Dimer Lyophilized Purified Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Relevance
A fibrin degradation fragment or product that is produced by the action of plasmin on fibrin in the clot dissolution process. -
Alternative names
- D Dimer
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human D-Dimer standard curve in Sample Diluent NS.The D-Dimer standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Example of human D-Dimer standard curve in Sample Diluent NS.The D-Dimer standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native D-Dimer in human serum, plasma (citrate), and plasma (heparin) samples.The concentrations of D-Dimer were measured in duplicates, interpolated from the D-Dimer standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1%, plasma (citrate) 10%, and plasma (heparin) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean D-Dimer concentration was determined to be 13.13 µg/mL in serum, 2.79 µg/mL in plasma (citrate), and 0.66 µg/ml (656.15 ng/mL) in plasma (heparin).
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Serum from ten individual healthy human mixed sex donors was measured in duplicate.One donor sample measured below the range of the assay. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean D-Dimer concentration was determined to be 9.46 µg/mL with a range of ND – 54.04 µg/mL.
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Linearity of dilution.Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Native D-Dimer was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.
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Linearity of dilution.Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
D-Dimer was spiked into the following biological samples and diluted in a 2-fold dilution series in Sample Diluent NS.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab260076 has been referenced in 1 publication.
- Sommerfeld O et al. Complement factor D is linked to platelet activation in human and rodent sepsis. Intensive Care Med Exp 9:41 (2021). PubMed: 34396466