Key features and details
- Sensitivity: 0.2 ng/ml
- Range: 0.205 ng/ml - 50 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman DAND5 ELISA Kit
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12%
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Range0.205 ng/ml - 50 ng/ml
Sample specific recovery Sample type Average % Range Serum 142.8 134% - 149% Plasma 128.6 92% - 144% Cell culture media 98.66 92% - 111%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Human DAND5 ELISA Kit is designed for the quantitative determination of DAND5 (COCO) in cell culture supernatants, plasma and serum samples.
Δ Note: Human DAND5 concentration is low in normal serum and plasma samples and may not be detectable in this assay.
This assay employs an antibody specific for human DAND5 coated on a 96-well plate. Standards and samples are pipetted into the wells and DAND5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human DAND5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of DAND5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
No cross-reactivity with human ActivinA, BMP4, DAN or mouse DAND5 is observed.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Anti-Human DAND5 coated Microplate (12 x 8 wells) 1 unit Assay Diluent C 1 x 30ml Biotinylated Anti-Human DAND5 Detection Antibody 2 vials Human DAND5 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml
FunctionSeems to play a role in the correct specification of the left-right axis. May antagonize NODAL and BMP4 signaling. Cystine knot-containing proteins play important roles during development, organogenesis, tissue growth and differentiation.
Sequence similaritiesBelongs to the DAN family.
Contains 1 CTCK (C-terminal cystine knot-like) domain.
- Information by UniProt
- BMP antagonist 3
- cerberus 2
ab267598 has not yet been referenced specifically in any publications.