Product nameHuman DAZAP1 knockout HEK293T cell lysate
See all DAZAP1 kits
Access thousands of knockout cell lysates, generated from commonly used cancer cell lines.
See here for more information on knockout cell lysates.
User storage instructions: After reconstitution, store the lysate at -80°C.
Parental Cell LineHEK293T
Mutation descriptionKnockout achieved by using CRISPR/Cas9, Homozygous: 8 bp deletion in exon 6.
Knockout validationSanger Sequencing, Western Blot (WB)
Reconstitution notesTo use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.
*Usage of SDS sample buffer is not recommended with these lyophilized lysates.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Tested applicationsSuitable for: WBmore details
Storage instructionsStore at -80°C. Please refer to protocols.
Components 1 kit ab260389 - Human DAZAP1 knockout HEK293T cell lysate (Lyophilized) 1 x 100µg ab255553 - Human Wild Type HEK293T cell lysate (Lyophilized) 1 x 100µg
STR AnalysisAmelogenin X D5S818: 8, 9 D13S317: 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 16, 19 TH01: 7, 9.3 TPOX: 11 CSF1PO: 11, 12
FunctionRNA-binding protein, which may be required during spermatogenesis.
Tissue specificityMainly expressed in testis. Expressed to a lower level in thymus. Weakly or not expressed in heart, liver, brain, placenta, lung, skeletal muscle, kidney and pancreas.
Sequence similaritiesContains 2 RRM (RNA recognition motif) domains.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm. Nucleus. Predominantly cytoplasmic (By similarity). Nuclear at some stages of spermatozoides development. In midpachytene spermatocytes, it is localized in both the cytoplasm and the nuclei and is clearly excluded from the sex vesicles. In round spermatids, it localizes mainly in the nuclei, whereas in elongated spermatids, it localizes to the cytoplasm.
- Information by UniProt
- DAZ associated protein 1
- DAZ-associated protein 1
KO cell lines
Our Abpromise guarantee covers the use of ab257912 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 43 kDa.|
Lane 1:Wild-type HEK293T cell lysate (20 ug)
Lane 2:DAZAP1 knockout HEK293T cell lysate (20 ug)
Lane 3:Jurkat cell lysate (20 ug)
Lane 4:HeLa cell lysate (20 ug)
ab184183 was shown to specifically react with DAZAP1 in wild-type HEK293T cells. The band observed in knockout cell line ab266469 (knockout cell lysate ab257912) lane below 43 kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and DAZAP1 knockout samples were subjected to SDS-PAGE. ab184183 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Homozygous: 8 bp deletion in exon 6
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab257912 has not yet been referenced specifically in any publications.