• Product name
    Human DcR2 ELISA Kit
    See all DcR2 kits
  • Detection method
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 10 pg/ml
  • Range
    5.487 pg/ml - 4000 pg/ml
  • Recovery

    97 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 98.44 90% - 107%
    Serum 99.99 91% - 107%
    Plasma 93.86 83% - 108%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s DcR2 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human DcR2 in serum, plasma and cell culture supernatants

    This assay employs an antibody specific for Human DcR2 coated on a 96-well plate. Standards and samples are pipetted into the wells and DcR2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human DcR2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of DcR2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform


  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human DcR2 2 vials
    DcR2 Microplate (12 x 8 wells) 1 unit
    Recombinant Human DcR2 Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas
  • Relevance
    DcR2 is a type 1 TNF R family membrane protein which is a receptor for TRAIL. In the Trail receptor family, DcR2 but also Trail R3 (DcR1) antagonize TRAIL-induced apoptosis whereas Trail R1 (DR4) and Trail R2 (DR5) transducer an apoptosis signal. DcR2 has a truncated intracellular domain. Overexpression of DcR2 has been shown to protect cells bearing Trail R1 and/or Trail R2 from Trail-induced apoptosis. Many tumor cell lines don’t express the decoy receptors and are therefore sensitive to TRAIL if they express Trail R1 and/or Trail R2.
  • Cellular localization
    Cell Membrane; single-pass type I membrane protein
  • Alternative names
    • CD264
    • CD264 antigen
    • DCR2
    • Decoy receptor 2
    • TNF related apoptosis inducing ligand receptor 4
    • TRAIL R4
    • TRAIL receptor 4
    • TRAIL receptor with a truncated death domain
    • TRAILR4
    • TRUNDD
    • Tumor necrosis factor receptor superfamily member 10d decoy with truncated death domain
    • Tumor necrosis factor receptor superfamily member 10D precursor
    see all
  • Database links


Our Abpromise guarantee covers the use of ab100657 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Representative standard curve using ab100657

  • Representative standard curve using ab100657



ab100657 has not yet been referenced specifically in any publications.

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