Overview

  • Product name
    Human DCR2 ELISA Kit (CD264)
    See all DcR2 kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Sample A 8 5723pg/ml 212 3.7%
    Sample B 8 3063pg/ml 218 7.1%
    Inter-assay
    Sample n Mean SD CV%
    Sample A 12 6531pg/ml 627 9.6%
    Sample B 12 3123pg/ml 369 11.8%
  • Sample type
    Serum, Cell culture extracts
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 64 pg/ml
  • Range
    312.5 pg/ml - 10000 pg/ml
  • Assay time
    3h 40m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s DCR2 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of DCR2 in Human serum, plasma, buffered solutions or supernatants.

    A monoclonal antibody specific for DCR2 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known DCR2 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for DCR2 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of DCR2 present in the samples.

    This kit will recognize both endogenous and recombinant Human DCR2.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
    Wash Buffer White 1 x 10ml 2 x 10ml
    Biotinylated anti DCR2 Red 1 x 400µl 2 x 400µl
    Biotinylated Antibody Diluent Red 1 x 7ml 1 x 13ml
    TMB Substrate 1 x 11ml 1 x 24ml
    DCR2 Microplate (12 x 8 well strips) 1 unit 2 units
    DCR2 Standard (lyophilized) Yellow 2 vials 4 vials
    HRP Diluent Red 1 x 23ml 1 x 23ml
    Stop Reagent Black 1 x 11ml 2 x 11ml
    Streptavidin-HRP 2 x 5µl 4 x 5µl
  • Research areas
  • Relevance
    DcR2 is a type 1 TNF R family membrane protein which is a receptor for TRAIL. In the Trail receptor family, DcR2 but also Trail R3 (DcR1) antagonize TRAIL-induced apoptosis whereas Trail R1 (DR4) and Trail R2 (DR5) transducer an apoptosis signal. DcR2 has a truncated intracellular domain. Overexpression of DcR2 has been shown to protect cells bearing Trail R1 and/or Trail R2 from Trail-induced apoptosis. Many tumor cell lines don’t express the decoy receptors and are therefore sensitive to TRAIL if they express Trail R1 and/or Trail R2.
  • Cellular localization
    Cell Membrane; single-pass type I membrane protein
  • Alternative names
    • CD264
    • CD264 antigen
    • DCR2
    • Decoy receptor 2
    • TNF related apoptosis inducing ligand receptor 4
    • TRAIL R4
    • TRAIL receptor 4
    • TRAIL receptor with a truncated death domain
    • TRAILR4
    • TRUNDD
    • Tumor necrosis factor receptor superfamily member 10d decoy with truncated death domain
    • Tumor necrosis factor receptor superfamily member 10D precursor
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab46017 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab46017

Protocols

References

This product has been referenced in:
  • Chen J  et al. High-dose HOOK effect in urinary DcR2 assay in patients with chronic kidney disease. Clin Biochem N/A:N/A (2018). ELISA ; Human . Read more (PubMed: 29879421) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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