Overview

  • Product name

    Human DcR3 ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type

    Serum, Plasma, Cell culture media
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    0.3 ng/ml
  • Range

    0.3 ng/ml - 80 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 129.9 107% - 144%
    Plasma 107.5 74% - 134%
    Cell culture media 141.9 122% - 150%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam's DcR3 Human ELISA Kit (ab193697) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human DcR3 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for human DcR3 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures DcR3 present in the samples. The wells are washed and biotinylated anti-human DcR3 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of DcR3 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    300X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent 1 x 15ml
    Biotinylated Human DcR3 detection antibody (lyophilized) 2 vials
    Human DcR3 Standards (lyophilized) 2 vials
    Pre-coated Human DcR3 Microplate (12 Strips x 8 wells) 1 unit
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Function

    Decoy receptor that can neutralize the cytotoxic ligands TNFS14/LIGHT, TNFSF15 and TNFSF6/FASL. Protects against apoptosis.
  • Tissue specificity

    Detected in fetal lung, brain and liver. Detected in adult stomach, spinal cord, lymph node, trachea, spleen, colon and lung. Highly expressed in several primary tumors from colon, stomach, rectum, esophagus and in SW480 colon carcinoma cells.
  • Sequence similarities

    Contains 4 TNFR-Cys repeats.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • DcR3
    • Decoy receptor 3
    • Decoy receptor for Fas ligand
    • M68
    • TNF6B_HUMAN
    • TNFRSF6B
    • Tumor necrosis factor receptor superfamily member 6B
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab193697 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

Protocols

References

ab193697 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab193697.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up