Human Decorin ELISA Kit (DCN) (ab99998)
Key features and details
- Sensitivity: 1.5 pg/ml
- Range: 0.96 pg/ml - 700 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Decorin ELISA Kit (DCN)
See all Decorin kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 1.5 pg/ml -
Range
0.96 pg/ml - 700 pg/ml -
Recovery
> 93 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 93.82 84% - 104% Serum 122.5 111% - 130% Plasma 111.8 103% - 119% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Decorin (DCN) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Decorin in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human Decorin coated on a 96-well plate. Standards and samples are pipetted into the wells and Decorin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti Human Decorin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Decorin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Notes
Optimisation may be required with urine samples.
The standard is affinity purified.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml Biotinylated anti-Human Decorin 2 vials Decorin Microplate (12 x 8 well strips) 1 unit Recombinant Human Decorin Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
May affect the rate of fibrils formation. -
Involvement in disease
Defects in DCN are the cause of congenital stromal corneal dystrophy (CSCD) [MIM:610048]. Corneal dystrophies are inherited, bilateral, primary alterations of the cornea that are not associated with prior inflammation or secondary to systemic disease. Most show autosomal dominant inheritance. -
Sequence similarities
Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.
Contains 12 LRR (leucine-rich) repeats. -
Post-translational
modificationsThe attached glycosaminoglycan chain can be either chondroitin sulfate or dermatan sulfate depending upon the tissue of origin. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Alternative names
- Bone proteoglycan II
- CSCD
- DCN
see all -
Database links
- Entrez Gene: 1634 Human
- Omim: 125255 Human
- SwissProt: P07585 Human
- Unigene: 728830 Human
Images
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab99998 has been referenced in 3 publications.
- Kim JS et al. Exercise in advanced prostate cancer elevates myokine levels and suppresses in-vitro cell growth. Prostate Cancer Prostatic Dis 25:86-92 (2022). PubMed: 35152272
- Iwanaga S et al. Hybrid Training System Improves Insulin Resistance in Patients with Nonalcoholic Fatty Liver Disease: A Randomized Controlled Pilot Study. Tohoku J Exp Med 252:23-32 (2020). PubMed: 32863329
- Ono YJ et al. Decorin induced by progesterone plays a crucial role in suppressing endometriosis. J Endocrinol 223:203-16 (2014). Human . PubMed: 25244916