Human Anti-Dengue virus IgG ELISA Kit (ab108728)

Submit an Abreview Q&A (7)

Overview

  • Product name
    Human Anti-Dengue virus IgG ELISA Kit
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Pos.Serum 8 4.34%
    Inter-assay
    Sample n Mean SD CV%
    Pos.Serum 8 6.76%
  • Sample type
    Serum, Heparin Plasma, Citrate Plasma
  • Assay type
    Indirect
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s anti-Dengue virus IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Dengue virus in Human serum and plasma.

     

    A 96-well plate has been precoated with Dengue virus antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Dengue virus-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Dengue virus IgG sample captured in plate.

  • Tested applications
    Suitable for: Indirect ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108728 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA Use at an assay dependent concentration.

Protocols

References

ab108728 has not yet been referenced specifically in any publications.

Publishing research using ab108728? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Submit a review Submit a question

7 reviews or Q&A

10
Votes: Highest First

I want to know about sample Serum, Plasma if my sample mix with EDTA. EDTA will effect with ELISA kit ?

i will use two kit for my sample.my sample is Serum, Plasma with EDTA

and abcam product i need is

Dengue Virus IgG ...

Read More



The Dengue IgG and IgM Kits have been validated for use on serum samples and plasma samples collected with citrate. Although it may well work we have not tried the kit with samples collected using EDTA. We would therefore not be able to guara...

Read More

Customer kindly contacted us regarding the specificity of ab108728 for Dengue Virus serotypes 1-4. He was specifically wondering if we had any data showing that the antibody in the kit recognizes all 4 serotypes.


I contacted the laboratory, and they confirmed that ab108728 does in fact cross react with Dengue Virus serotypes 1-4. It is very difficult to get samples with defined subtype of Dengue because the antibodies show a strong cross reactivity. Due to...

Read More

Does this ELISA kit able to detect antibody against all 4 serotypes? I believe plate is coated with DEN-2 antigen but I wonder if there is cross reactivity.

This product is not in stock and currently has a 1 -2 week lead time. The kit will recognize all four serotypes (DEN-1, DEN-2, DEN-3, and DEN-4). While the Dengue Virus IgG ELISA is coated with purified Dengue 2 Virus proteins from strain 16681. Dengue...

Read More

Is it known whether this kit will cross react with West Nile virus too?

-Is it specific to Dengue type 2?

The Dengue Virus IgG ELISA is coated with purified Dengue 2 Virus proteins from strain 16681. Dengue Virus Type 2 is cross reactive with antibodies from Serotypes 1-4, thus the kit will detect IgG from patient samples past infected with any of the four...

Read More

Might you be able to provide information on the specific anti-Dengue antibody used as capture antibody in the ELISA, particularly the immunogenic sequence (ie sequence injected into mouse or rabbit to produce the Ab?)



In this assay, microtiter strip wells are precoated with Dengue Virus antigens type 2 to bind corresponding antibodies of the specimen. No capture antibody is used. The only antibody in the kit is the anti-human-HRP conjugate used to detect a...

Read More

Is it known whether this kit will cross react with West Nile virus too?

We do not have cross reactivity data for West Nile Virus.

Hola, quisiera saber que proteina usais para los kits comerciales, ya que veo que hay la posibilidad de la NS1 y la enveloped protein (para los 4 serotipos de virus). Muchas gracias anticipadas

Te pido disculpas por la espera. Tenemos en el catálogo dos kits para determinar el virus Dengue en función del isotipo. Éstos son: ab108728 y ab108729. En ambos kits las placas están cubiertas con antígenos de virus 2 de la cepa 16681. Espero haberte ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up