Human Anti-Dengue virus IgG ELISA Kit (ab108728)

Submit a review Q&A (7)References (2)
Serologic ELISA assay principle

    Key features and details

    • Sample type: Cit plasma, Hep Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Indirect
    • Reacts with: Human

    Overview

    • Product name

      Human Anti-Dengue virus IgG ELISA Kit

    • Detection method

      Colorimetric

    • Precision

      Intra-assay
      Sample n Mean SD CV%
      Pos.Serum 8 4.34%
      Inter-assay
      Sample n Mean SD CV%
      Pos.Serum 8 6.76%

    • Sample type

      Serum, Hep Plasma, Cit plasma

    • Assay type

      Indirect

    • Assay duration

      Multiple steps standard assay

    • Species reactivity

      Reacts with: Human

    • Product overview

      Dengue virus ELISA kit - IgG is designed for the accurate qualitative measurement of IgM class antibodies against Dengue virus in Human serum and plasma.


      A 96-well plate has been precoated with Dengue virus antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Dengue virus-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Dengue virus IgG sample captured in plate.

    • Platform

      Microplate

    Properties

    Images

    • Serologic ELISA assay principle
      Serologic ELISA assay principle
      Specific antigens are coated on the 96-well plate, controls or test samples are added to the well and incubated. The wells are washed to remove any unbound Human anti-antigen antibodies (Ig). A horseradish peroxidase (HRP) labelled anti-Human Ig conjugate is added to the wells. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Human anti-antigen Ig captured on the plate.

    References (2)

    Publishing research using ab108728? Please let us know so that we can cite the reference in this datasheet.

    ab108728 has been referenced in 2 publications.

    • Lin CY  et al. High levels of serum hyaluronan is an early predictor of dengue warning signs and perturbs vascular integrity. EBioMedicine 48:425-441 (2019). PubMed: 31526718
    • Montoya-Ruiz C & Rodas JD Epidemiological Surveillance of Viral Hemorrhagic Fevers With Emphasis on Clinical Virology. Methods Mol Biol 1604:55-78 (2018). ELISA ; Human . PubMed: 28986825

    Customer reviews and Q&As

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    1-7 of 7 Abreviews or Q&A

    Question

    I want to know about sample Serum, Plasma if my sample mix with EDTA. EDTA will effect with ELISA kit ?

    i will use two kit for my sample.my sample is Serum, Plasma with EDTA

    and abcam product i need is

    Dengue Virus IgG Human ELISA Kit (ab108728)

    Dengue Virus IgM Human ELISA Kit (ab108729)

    Please you advise me.Because i will order abcam soon and i afraid EDTA will effect to Kit.

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    Answer



    The Dengue IgG and IgM Kits have been validated for use on serum samples and plasma samples collected with citrate. Although it may well work we have not tried the kit with samples collected using EDTA. We would therefore not be able to guarantee the performance of the kit with these samples.

    If you would like to purchase either ab108728 or ab108729 and use it with your serum prepared with EDTA, you would be eligible to participate in the Abtrial scheme. This offer means that if you purchase ab108728 or ab108729 as normal, test the kit with your samples in and let us know of the results through an Abreview (no matter whether positive or negative) you would then be eligible to claim the value of ab108728 or ab108729 off any product in our catalogue. More information on this offer can be found here:

    www.abcam.com/abtrial

    Please note that the antibody to be tested must be purchased, tested, the Abreview submitted and the free product claimed within a 4 month period.

    If you would be interested in participating in this scheme please do let me know as a discount code needs to be issued prior to the purchase of ab108728 or ab108729.

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    Question

    Customer kindly contacted us regarding the specificity of ab108728 for Dengue Virus serotypes 1-4. He was specifically wondering if we had any data showing that the antibody in the kit recognizes all 4 serotypes.

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    Answer


    I contacted the laboratory, and they confirmed that ab108728 does in fact cross react with Dengue Virus serotypes 1-4. It is very difficult to get samples with defined subtype of Dengue because the antibodies show a strong cross reactivity. Due to this cross reactivity it is sufficient to use Dengue-2 antigen (the antigen used in ab108728) to detect antibodies against subtypes 1-4. I have attached an extract of a WHO publication. On page 100 of the original document you will find the related statement in the paragraph right above the figure.

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    Question

    Does this ELISA kit able to detect antibody against all 4 serotypes? I believe plate is coated with DEN-2 antigen but I wonder if there is cross reactivity.

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    Answer

    This product is not in stock and currently has a 1 -2 week lead time. The kit will recognize all four serotypes (DEN-1, DEN-2, DEN-3, and DEN-4). While the Dengue Virus IgG ELISA is coated with purified Dengue 2 Virus proteins from strain 16681. Dengue Virus Type 2 is cross reactive with antibodies from Serotypes 1-4.

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    Question

    Is it known whether this kit will cross react with West Nile virus too?

    -Is it specific to Dengue type 2?

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    Answer

    The Dengue Virus IgG ELISA is coated with purified Dengue 2 Virus proteins from strain 16681. Dengue Virus Type 2 is cross reactive with antibodies from Serotypes 1-4, thus the kit will detect IgG from patient samples past infected with any of the four serotypes. The kit is not specific to Dengue Type 2.

    It has not been tested for reactivity to west nile virus.

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    Question

    Might you be able to provide information on the specific anti-Dengue antibody used as capture antibody in the ELISA, particularly the immunogenic sequence (ie sequence injected into mouse or rabbit to produce the Ab?)

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    Answer



    In this assay, microtiter strip wells are precoated with Dengue Virus antigens type 2 to bind corresponding antibodies of the specimen. No capture antibody is used. The only antibody in the kit is the anti-human-HRP conjugate used to detect any antibody bound to the Dengue Virus antigens type 2.

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    Question

    Is it known whether this kit will cross react with West Nile virus too?

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    Answer

    We do not have cross reactivity data for West Nile Virus.

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    Question

      Hola, quisiera saber que proteina usais para los kits comerciales, ya que veo que hay la posibilidad de la NS1 y la enveloped protein (para los 4 serotipos de virus). Muchas gracias anticipadas

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    Answer

    Te pido disculpas por la espera. Tenemos en el catálogo dos kits para determinar el virus Dengue en función del isotipo. Éstos son: ab108728 y ab108729. En ambos kits las placas están cubiertas con antígenos de virus 2 de la cepa 16681. Espero haberte ayudado. No dudes en contactarnos para cualquier otra duda.

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