Overview

  • Product name

    Human Anti-Dengue virus IgM ELISA Kit
    See all Dengue Virus IgM kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Pos.Serum 7 3.1%
    Inter-assay
    Sample n Mean SD CV%
    Pos.Serum 20 4.8%
  • Sample type

    Serum, Heparin Plasma, Citrate Plasma
  • Assay type

    Indirect
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Dengue virus ELISA kit - IgM is designed for the accurate qualitative measurement of IgM class antibodies against Dengue virus in Human serum and plasma.


     A 96-well plate has been precoated with Dengue virus antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgM conjugate is added to the wells, which binds to the immobilized Dengue virus-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Dengue virus IgM sample captured in plate.

  • Tested applications

    Suitable for: Indirect ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA Use at an assay dependent concentration.

Protocols

References

This product has been referenced in:

  • Gunisetty S  et al. Analysis of dengue specific memory B cells, neutralizing antibodies and binding antibodies in healthy adults from India. Int J Infect Dis N/A:N/A (2019). ELISA ; Human . Read more (PubMed: 30658170) »
  • Montoya-Ruiz C & Rodas JD Epidemiological Surveillance of Viral Hemorrhagic Fevers With Emphasis on Clinical Virology. Methods Mol Biol 1604:55-78 (2018). ELISA ; Human . Read more (PubMed: 28986825) »
See all 2 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer



The Dengue IgG and IgM Kits have been validated for use on serum samples and plasma samples collected with citrate. Although it may well work we have not tried the kit with samples collected using EDTA. We would therefore not be able to guarantee the performance of the kit with these samples.

If you would like to purchase either ab108728 or ab108729 and use it with your serum prepared with EDTA, you would be eligible to participate in the Abtrial scheme. This offer means that if you purchase ab108728 or ab108729 as normal, test the kit with your samples in and let us know of the results through an Abreview (no matter whether positive or negative) you would then be eligible to claim the value of ab108728 or ab108729 off any product in our catalogue. More information on this offer can be found here:

www.abcam.com/abtrial

Please note that the antibody to be tested must be purchased, tested, the Abreview submitted and the free product claimed within a 4 month period.

If you would be interested in participating in this scheme please do let me know as a discount code needs to be issued prior to the purchase of ab108728 or ab108729.

Read More

Answer

Thank you for your enquiry.

I would appreciate if you could confirm the productcode for the kit you mention. Is this ab108729
Dengue Virus IgM Human ELISA Kit?

For ab108729, this kit is tested and guaranteed to be used as an ELISA kit with the wells already provided. The wells are pre coated with coating antibody as described on the datasheet. Therefore, regrettably scaling down to micro ELISA using smaller wells or another platform would not be possible.

I am sorry this is not better news for you on this occasion and there are no other suitable products to suggest. If you have any further questions, please do not hesitate to contact us.

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Answer

Thank you for contacting us.

To further confirm the specificity of this kit. It will be suitable for detection of all the serotypes of Dengue Virus.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Answer

Thank you for contacting us.

The best suitable product we can offer is ab108729;

https://www.abcam.com/Dengue-Virus-IgM-Human-ELISA-Kit-ab108729.html

Please compare the datasheets of the products.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Answer

Te pido disculpas por la espera. Tenemos en el catálogo dos kits para determinar el virus Dengue en función del isotipo. Éstos son: ab108728 y ab108729. En ambos kits las placas están cubiertas con antígenos de virus 2 de la cepa 16681. Espero haberte ayudado. No dudes en contactarnos para cualquier otra duda.

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