Overview

  • Product name

    Human DKK3 ELISA Kit
    See all Dkk3 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 5 3.3%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 3.8%
  • Sample type

    Cell culture supernatant, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    34.1 pg/ml
  • Range

    156 pg/ml - 10000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 102 93% - 118%
    Cell culture media 91 84% - 96%
    Heparin Plasma 97 91% - 107%
    EDTA Plasma 91 90% - 103%
    Citrate Plasma 103 97% - 109%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    DKK3 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of DKK3 protein in human serum, plasma, and cell culture supernatant samples


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB Development Solution is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    DKK3 is a member of the dickkopf family, and is involved in the Wnt signaling pathway during embryonic development. In adults, DKK3 is implicated in bone formation. Down regulation of DKK3 expression is seen in a variety of cell cancer lines.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent CPI 1 x 6ml
    10X Human DKK3 Capture Antibody 1 x 600µl
    10X Human DKK3 Detector Antibody 1 x 600µl
    Human DKK3 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
  • Tissue specificity

    Highest expression in heart, brain, and spinal cord.
  • Sequence similarities

    Belongs to the dickkopf family.
  • Domain

    The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.
  • Post-translational
    modifications

    N- and O-glycosylated.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • Dickkopf 3
    • Dickkopf 3 homolog
    • Dickkopf 3 homolog (Xenopus laevis)
    • Dickkopf homolog 3
    • Dickkopf related protein 3
    • Dickkopf related protein 3 Precursor
    • Dickkopf WNT signaling pathway inhibitor 3
    • Dickkopf, Xenopus, homolog of, 3
    • Dickkopf-3
    • Dickkopf-related protein 3
    • Dickkopf3
    • DKK 3
    • Dkk-3
    • DKK3
    • DKK3_HUMAN
    • hDkk 3
    • hDkk-3
    • hDkk3
    • Regulated in glioma
    • REIC
    • RIG
    • RIG-like 5-6
    • RIG-like 7-1
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab236710 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • The DKK3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of DKK3 were measured in duplicates, interpolated from the DKK3 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 20%, plasma (citrate) 10%, plasma (heparin) 10%, and plasma (EDTA) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean DKK3 concentration was determined to be 38.4 ng/mL in serum, 30.5 ng/mL in plasma (citrate), 29.6 ng/ml in plasma (heparin), and 29.0 ng/mL in plasma (EDTA).

  • The concentrations of DKK3 were measured in duplicates, interpolated from the DKK3 standard curves and corrected for sample dilution. Undiluted samples are as follows: HUVEC supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean DKK3 concentration was determined to be 19.8 ng/mL in HUVEC supernatant.

  • Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean DKK3 concentration was determined to be 40.4 ng/mL with a range of 27.3 – 65.8 ng/mL.

Protocols

References

ab236710 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab236710.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up