Overview

  • Product name

    Human DKK4 ELISA Kit
    See all DKK4 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    supernatant 5 7.4%
    Inter-assay
    Sample n Mean SD CV%
    supernatant 3 2.9%
  • Sample type

    Cell culture supernatant, Serum, EDTA Plasma, Cit plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    5.17 pg/ml
  • Range

    21.88 pg/ml - 1400 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 110 103% - 115%
    Serum 83 82% - 86%
    EDTA Plasma 92 87% - 98%
    Cit plasma 99 95% - 105%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
    Does not react with: Cow
  • Product overview

    DKK4 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of DKK4 protein in human serum, plasma (citrate), plasma (EDTA), and cell culture supernatant samples


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB Development Solution is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    DKK4 is a member of the dickkopf-related protein family. DKK4 is involved during development through Wnt signaling. In adults, DKK4 is involved in bone formation, cancer, and Alzheimer disease.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human DKK4 Capture Antibody 1 x 600µl
    10X Human DKK4 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml
    Human DKK4 Lyophilized recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
  • Tissue specificity

    Expressed in cerebellum, T-cells, esophagus and lung.
  • Sequence similarities

    Belongs to the dickkopf family.
  • Domain

    The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.
  • Post-translational
    modifications

    Appears to be not glycosylated.
    Can be proteolytically processed by a furin-like protease.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • Dickkopf 4
    • Dickkopf gene 4
    • Dickkopf homolog 4
    • Dickkopf homolog 4 (Xenopus laevis)
    • Dickkopf related protein 4
    • Dickkopf-4
    • Dickkopf-related protein 4 short form
    • Dickkopf4
    • DKK 4
    • Dkk-4
    • DKK4
    • DKK4_HUMAN
    • hDkk 4
    • hDkk-4
    • hDkk4
    • MGC129562
    • MGC129563
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab243690 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Standard Curve comparison between human DKK4 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

  • The DKK4 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of DKK4 were measured in duplicates, interpolated from the DKK4 standard curves and corrected for sample dilution. Undiluted samples are as follows: SW480 supernatant 100 %. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean DKK4 concentration was determined to be 1,245 pg/mL in SW480 supernatant.

  • Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean DKK4 concentration was determined to be 61.9 pg/mL with a range of ND – 97.5 pg/mL. ND = not detected, below the range of the assay.

  • Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean DKK4 concentration was determined to be 43.0 pg/mL with a range of ND – 113.2 pg/mL. ND = not detected, below the range of the assay.

Protocols

References

ab243690 has not yet been referenced specifically in any publications.

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