Overview

  • Product name

    Human DLL1 ELISA Kit
    See all DLL1 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type

    Serum, Plasma, Cell culture media
  • Assay type

    Sandwich
  • Sensitivity

    45 pg/ml
  • Range

    45 pg/ml - 12000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 100.2 92% - 108%
    Plasma 87.96 74% - 96%
    Cell culture media 90.16 82% - 97%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam's DLL1 Human ELISA Kit (ab193698) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human DLL1 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for human DLL1 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures DLL1 present in the samples. The wells are washed and biotinylated anti-human DLL1 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of DLL1 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated Human DLL-1 detection antibody (lyophilized) 2 vials
    Human DLL-1 standards (lyophilized) 2 vials
    Pre-coated Human DLL-1 Microplate (12 strips x 8 wells) 1 unit
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Function

    Acts as a ligand for Notch receptors. Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor.
  • Tissue specificity

    Expressed in heart and pancreas, with lower expression in brain and muscle and almost no expression in placenta, lung, liver and kidney.
  • Sequence similarities

    Contains 1 DSL domain.
    Contains 8 EGF-like domains.
  • Post-translational
    modifications

    Ubiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Alternative names

    • Delta-like protein 1
    • Delta1
    • DLL1
    • DLL1_HUMAN
    • Drosophila Delta homolog 1
    • H-Delta-1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab193698 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

Protocols

References

ab193698 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab193698.
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