Overview

  • Product name
    Human Doublecortin Antibody Pair - BSA and Azide free
    See all Doublecortin kits
  • Detection method
    Colorimetric
  • Assay type
    ELISA set
  • Range
    15.63 pg/ml - 1000 pg/ml
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Product overview

    Antibody Pairs – BSA and Azide free include a capture and a detector antibody pair suitable for sandwich ELISA. The Antibody Pair can be used to quantify native and recombinant Human Doublecortin.


    For additional information on the performance of the antibody pair used in this kit, please see equivalent SimpleStep ELISA® Kit (ab218267), which use the same antibody pair. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.


    Please note that the range provided is only an estimation based on the performance of a related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay.


    To receive an electronic copy of the Certificate of Analysis, please send an email with "CoA for matched antibody pair kit" in the subject line and the desired product number and lot number in the body of the email.


    Download SDS here.


     

  • Tested applications
    Suitable for: ELISAmore details
  • Platform
    Reagents

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 10 x 96 tests
    Human Doublecortin Capture Antibody 1 x 100µg
    Human Doublecortin Detector Antibody 1 x 100µg
  • Research areas
  • Function
    Seems to be required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCAMKL1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with LIS-1 of an overlapping, but distinct, signaling pathways that promote neuronal migration.
  • Tissue specificity
    Highly expressed in neuronal cells of fetal brain (in the majority of cells of the cortical plate, intermediate zone and ventricular zone), but not expressed in other fetal tissues. In the adult, highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas.
  • Involvement in disease
    Defects in DCX are the cause of lissencephaly X-linked type 1 (LISX1) [MIM:300067]; also called X-LIS or LIS. LISX1 is a classic lissencephaly characterized by mental retardation and seizures that are more severe in male patients. Affected boys show an abnormally thick cortex with absent or severely reduced gyri. Clinical manifestations include feeding problems, abnormal muscular tone, seizures and severe to profound psychomotor retardation. Female patients display a less severe phenotype referred to as 'doublecortex'.
    Defects in DCX are the cause of subcortical band heterotopia X-linked (SBHX) [MIM:300067]; also known as double cortex or subcortical laminar heterotopia (SCLH). SBHX is a mild brain malformation of the lissencephaly spectrum. It is characterized by bilateral and symmetric plates or bands of gray matter found in the central white matter between the cortex and cerebral ventricles, cerebral convolutions usually appearing normal.
    Note=A chromosomal aberration involving DCX is found in lissencephaly. Translocation t(X;2)(q22.3;p25.1).
  • Sequence similarities
    Contains 2 doublecortin domains.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Alternative names
    • DBCN
    • Dbct
    • DC
    • DCX
    • DCX_HUMAN
    • Doublecortex
    • Doublin
    • FLJ51296
    • Lis X
    • Lis-X
    • Lissencephalin X
    • Lissencephalin-X
    • Lissencephaly X linked
    • Lissencephaly X linked doublecortin
    • LISX
    • Neuronal migration protein doublecortin
    • OTTHUMP00000023859
    • OTTHUMP00000023860
    • OTTHUMP00000216315
    • OTTHUMP00000216316
    • SCLH
    • XLIS
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab243995 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

References

ab243995 has not yet been referenced specifically in any publications.

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