• Product name

    Human DR4 ELISA Kit (CD261)
  • Detection method

  • Precision

    Sample n Mean SD CV%
    A 8 475pg/ml 13 2.76%
    B 8 54pg/ml 4 7.57%
    Sample n Mean SD CV%
    A 12 498pg/ml 40 8.11%
    B 12 53pg/ml 7 13.87%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 8 pg/ml
  • Range

    31.25 pg/ml - 1000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 93.4 250pg/ml - 1000pg/ml

  • Assay time

    2h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s DR4 (CD261) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of DR4 in serum, plasma, buffered solutions and cell culture media.

    A monoclonal antibody specific for DR4 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known DR4 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for DR4 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of DR4 present in the samples.

    This kit will recognize both endogenous and recombinant Human DR4.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform



  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
    200X Wash Buffer White 1 x 10ml 2 x 10ml
    Biotinylated Antibody Diluent Red 1 x 7ml 1 x 13ml
    Biotinylated anti-DR4 Red 1 x 400µl 2 x 400µl
    Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
    DR4 Microplate (12 x 8 well strips) 1 unit 2 units
    DR4 Standard (lyophilized) Yellow 2 vials 4 vials
    HRP Diluent Red 1 x 23ml 1 x 23ml
    Stop Reagent Black 1 x 11ml 2 x 11ml
    Streptavidin-HRP 2 x 5µl 4 x 5µl
  • Research areas

  • Relevance

    Human Trail R1, CD261 or DR4 (Death Receptor 4) is a TNF receptor, which is a receptor for Trail (APO2 ligand). DR4 is involved in apoptosis. In the Trail receptor family, DR4, but also Trail R2 (DR5) transduce an apoptosis signal, whereas Trail R3 (DcR1) and Trail R4 (DcR2) antagonize TRAIL-induced apoptosis. Binding of trimeric Trail to DR4 induces apoptosis by oligomerization (likely to be trimerization) of the receptor. DR4 cDNA encodes a 468 amino acid residue precursor protein containing extracellular cysteine-rich domains, a transmembrane domain and a cytoplasmic death domain. DR4 shares 55% of its amino acid sequence with Trail R2. DR4 is widely expressed in normal tissue.
  • Cellular localization

    Cell Membrane; single-pass type I membrane protein
  • Alternative names

    • APO2
    • CD261
    • Death receptor 4
    • TNFRSF10A
    • TRAIL receptor 1
    • TRAILR1
    see all
  • Database links


Our Abpromise guarantee covers the use of ab46510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Representative Standard Curve using ab46510



ab46510 has not yet been referenced specifically in any publications.

Customer reviews and Q&As


Thank you for contacting us.

A standard lysis buffer (such as RIPA buffer) can be used to prepare the cell lysates and the use protease inhibitor is indeed recommended.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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