Description

Associated products

Specifications

Our Abpromise guarantee covers the use of ab14946 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking - Blocking peptide for Anti-EEA1 antibody - Early Endosome Marker (ab2900)

  • Form

    Liquid
  • Additional notes

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

General Info

  • Alternative names

    • Early endosome antigen 1
    • Early endosome antigen 1, 162kD
    • Early endosome associated protein
    • EEA 1
    • EEA1
    • EEA1_HUMAN
    • Endosome associated protein p162
    • Endosome-associated protein p162
    • MST105
    • MSTP105
    • ZFYVE2
    • Zinc finger FYVE domain containing protein 2
    • Zinc finger FYVE domain-containing protein 2
    see all
  • Function

    Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.
  • Sequence similarities

    Contains 1 C2H2-type zinc finger.
    Contains 1 FYVE-type zinc finger.
  • Domain

    The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions.
  • Cellular localization

    Cytoplasm. Early endosome membrane.
  • Information by UniProt

Images

  • All lanes : Anti-EEA1 antibody - Early Endosome Marker (ab2900) at 1/500 dilution

    Lane 1 : HEK293 Whole Cell lysate
    Lane 2 : HEK293 Whole Cell lysate with Human EEA1 peptide (ab14946) at 1 µg

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

    Performed under reducing conditions.

    Observed band size: 180 kDa
    why is the actual band size different from the predicted?

References

ab14946 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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