• Product name
    Human EGF ELISA Kit
  • Detection method
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 1 pg/ml
  • Range
    0.82 pg/ml - 200 pg/ml
  • Recovery

    95 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 95.52 85% - 106%
    Serum 94.52 83% - 104%
    Plasma 93.76 84% - 105%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s EGF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human EGF in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human EGF coated on a 96-well plate. Standards and samples are pipetted into the wells and EGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human EGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of EGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimisation may be required with urine samples.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform


  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    600X HRP-Streptavidin Concentrate 1 x 200µl
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human EGF (lyophilized) 2 vials
    EGF Microplate (12 strips x 8 wells) 1 unit
    Recombinant Human EGF Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas
  • Relevance
    Epidermal growth factor (EGF) has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells. The EGF precursor is believed to exist as a membrane bound molecule which is proteolytically cleaved to generate the 53 amino acid peptide hormone that stimulates cells to divide. EGF exerts its actions by binding to the EGFR, a 170 kDa protein. Epidermal growth factor (EGF) is a key growth factor regulating cell survival. Through its binding to cell surface receptors, EGF activates an extensive network of signal transduction pathways that include activation of the PI3K/AKT, RAS/ERK and JAK/STAT pathways. Because of its key role in driving the proliferation of cells, EGFR is a target of several anti-cancer drugs currently in development.
  • Alternative names
    • Beta urogastrone
    • Epidermal growth factor
    • HOMG4
    • OTTHUMP00000219721
    • OTTHUMP00000219722
    • Pro epidermal growth factor
    • URG
    • Urogastrone
    see all
  • Database links


Our Abpromise guarantee covers the use of ab100504 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Standard curve in Diluent A : mean of duplicates (+/-SD)

  • Standard curve in Diluent B : mean of duplicates (+/-SD)



This product has been referenced in:
  • Kim SW  et al. Amniotic mesenchymal stem cells enhance wound healing in diabetic NOD/SCID mice through high angiogenic and engraftment capabilities. PLoS One 7:e41105 (2012). Human . Read more (PubMed: 22815931) »

See 1 Publication for this product

Customer reviews and Q&As

I am sorry to read that the most recent order of the EGF ELISA gave a poor result compared to previous kits. I have arranged for a free replacement from a different lot, order ***.

To check the status of the order please contact our Customer...

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Thank you for confirming these details and for your update. I am pleased to hear the FOCR kit has worked well. This enquiry has been forwarded to me as my colleague #### is currently away from the office.

As requested, I have now issued the ...

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Thank you for confirming that information.

I have now arranged for the kit to be sent out to you. The order number is xxxxx(Purchase order number FOCR xxxxx).

I hope this kit will work as your previous ones have, however, if you hav...

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Thank you for providing me with that information.

Could you please check how much HRP-streptavidin is in the unused kit which you have? Also, for both of these kits, what dilution did the protocol sent witheach kit advise?

Many than...

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We may have identified what the difference may be. Could you confirm, how did you dilute the HRP-streptavidin concentrate provided with the kit?

We have recently modified this reagent and the old kit contained 8 uL of 30,000x HRP-st...

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Thank you for providing that information.

I am sorry that it seems that this kit is not performing as expected. It would be helpful for me if you could provide me with the lot numbers or order numbers of the kits which you have found to work ...

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Thank you for contacting us and reporting the problems you have been encountering with the EGF Human ELISA Kit (ab100504). We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.


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Thank you for your enquiry. I am sorry to confirm that  Bovine samples have not been tested and guaranteed with the ab100504 EGF Human ELISA Kit,  so there is no cross reactivity data.  Epitope mapping was also not performed so we do not know exact...

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Thanks for your call tyesterday and for your patience while I have been in touch with the lab. I have heard back about your enquiry. Bovine samples have not been tested with the kit so we do not have any cross-reactivity data to conclusively determine ...

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