Question (29805) | Human EGF ELISA Kit (ab100504)

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Question

Hi I recently purchased the EGF human ELISA kit (ab100504). While I am aware that the kit is intended for detection of EGF in human samples, I was wondering if you knews the cross reactivity of the assay in other species such as Bovine. In other words, would the assay detect EGF in foetal bovine serum if used? Also, would you happen to know if the detection antibody used for EGF is monoclonal or polyclonal? Do you know if the detection antibody may have a immunogenecity to bind to a bovine epitope? Could you provide us with its structure so I can search? Thanks

Answer

Thank you for your enquiry. I am sorry to confirm that  Bovine samples have not been tested and guaranteed with the ab100504 EGF Human ELISA Kit,  so there is no cross reactivity data.  Epitope mapping was also not performed so we do not know exactly where the antibodies are binding.   However, we do know that the antibodies in this kit recognize the mature form of EGF. The human and bovine EGF reportedly share between 59% and 66% homology.  This percentage is not that high and it is likely the antibody would not detect in bovine samples, or would detect very weakly. The capture antibody is a monoclonal and the detection antibody polyclonal. I hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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