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Many thanks for the response. The kit I used contained ONLY 8 uL of the 30,000 HRP-streptavidin unlike the 200 uL you suggest it may have had, since it had only 8 uL I went for the 30,000 dilution as with the old protocol.
Asked on Apr 02 2012
Thank you for providing me with that information.
Could you please check how much HRP-streptavidin is in the unused kit which you have? Also, for both of these kits, what dilution did the protocol sent witheach kit advise?
Many thanks for your continued cooperation.
Answered on Apr 02 2012