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We purchased an ELISA kit for EGF (ab100504) a month ago (arrived 28th Sep 2012). I recently used the kit with the provided standards and my samples only to find that there was absolutely no signal whatsoever including the standard wells. I have in the past purchased the same kit and they have worked marvelously. Just to be absolutely certain, I repeated the assay with known EGF standards from a previous batch (which I knew worked and samples I knew gave me +ve results), only to get no signal whatsoever. I am unsure if this is a problem with the plate or the reagents.
Asked on Oct 23 2012
I am sorry to read that the most recent order of the EGF ELISA gave a poor result compared to previous kits. I have arranged for a free replacement from a different lot, order ***.
To check the status of the order please contact our Customer Service team and reference this number.
We have not received other complaints, aside from the complaint from your lab earlier in the year regarding a precipitate in the standards. I am not sure which reagent is defective in this most recent kit, but it is apparently one of the detection reagents, if the older standards that you tested are still intact and undegraded.
Please let us know if you have other questions or concerns.
Answered on Oct 23 2012