Overview

  • Product name

    Human EGF ELISA Kit
    See all EGF kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Human serum 5 8%
    Inter-assay
    Sample n Mean SD CV%
    Human serum 3 7%
  • Sample type

    Cell culture supernatant, Saliva, Milk, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    1.04 pg/ml
  • Range

    1.562 pg/ml - 100 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Saliva 92 90% - 96%
    Milk 96 81% - 121%
    Urine 103 101% - 105%
    Serum 108 93% - 122%
    Cell culture media 91 88% - 95%
    Hep Plasma 109 104% - 114%
    EDTA Plasma 116 104% - 127%
    Cit plasma 112 100% - 126%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
    Does not react with: Cow
  • Product overview

    Human EGF SimpleStep ELISA® kit (ab179888) has been re-developed with new capture and detector antibodies.This new kit has the same name but a different product number (ab217772).We have identified new recombinant monoclonal antibodies to use in the SimpleStep ELISA platform that provide a higher sensitivity when quantifying EGF in human serum,cell culture media, urine, saliva, milk, citrate plasma, heparin plasma and EDTA plasma.


    EGF in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of EGF protein in human serum, plasma, urine, saliva, milk, and cell culture supernatant samples.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Senstitivity:


    Samples in Sample Diluent NS - 1.04 pg/mL


    Samples in Sample Diluent 50BP - 1.14 pg/mL

  • Notes

    EGF precursor is a large 1185 amino acid group 1 member of the EGF family growth factors. All EGF family growth factors are synthesized as transmembrane proteins that undergo proteolytic processing to generate soluble forms. Human EGF is a 53 amino acid long fragment of the extracellular domain of EGF precursor. As other group 1 members, EGF binds to, and activates the EGF receptor. This promotes EGFR dimerization. EGF has variety of diverse physiological effects. EGF activity is complicated by the fact that other EGF family members utilize EGF receptor. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. EGF is proposed to affect growth and/or differentiation of many other fetal and adult tissues.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human EGF Capture Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 4BI 1 x 6ml
    Human EGF Detector Antibody (Lyophilized) 1 vial
    Human EGF Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent 50BP 1 x 20ml
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).
  • Tissue specificity

    Expressed in kidney, salivary gland, cerebrum and prostate.
  • Involvement in disease

    Hypomagnesemia 4
  • Sequence similarities

    Contains 9 EGF-like domains.
    Contains 9 LDL-receptor class B repeats.
  • Post-translational
    modifications

    O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Alternative names

    • Beta urogastrone
    • beta-urogastrone
    • EGF
    • EGF_HUMAN
    • Epidermal growth factor
    • HOMG4
    • OTTHUMP00000219721
    • OTTHUMP00000219722
    • Pro epidermal growth factor
    • URG
    • Urogastrone
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab217772 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of EGF were measured in duplicates, interpolated from the EGF standard curves and corrected for sample dilution. Undiluted samples are as follows: saliva 2.5%, urine 0.4% and serum 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EGF concentration was determined to be 5377 pg/mL in neat saliva, 25147 pg/mL in neat urine, and 101 pg/mL in neat serum.

  • The concentrations of EGF were measured in duplicates, interpolated from the EGF standard curves and corrected for sample dilution. Undiluted samples are as follows: whole milk 100%, de-fatted milk 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EGF concentration was determined to be 18,596 pg/mL in neat whole milk and 19,757 pg/mL in neat de-fatted milk.

  • Interpolated concentrations of a 200 pg/mL recombinant EGF spike in human platelet poor plasma citrate, platelet poor plasma heparin, platelet poor plasma EDTA, serum, and a 100 pg/mL recombinant EGF spike in RPMI +10% FBS media samples. The concentrations of EGF were measured in duplicates, interpolated from the EGF standard curves and corrected for sample dilution. Undiluted samples are as follows: plasma citrate 50%, plasma heparin 50%, plasma EDTA 50%, serum 50%, and RPMI + 10% FBS 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EGF concentration was determined to be 200 pg/mL with a range of 5.3 – 1436 pg/mL.

Protocols

References

ab217772 has not yet been referenced specifically in any publications.

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