Human EGFR Phosphorylation Antibody Array - Membrane (17 Targets) (ab134005)
Overview
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Product name
Human EGFR Phosphorylation Antibody Array - Membrane (17 Targets) -
Sample type
Tissue Extracts, Cell Lysate -
Assay type
Semi-quantitative -
Species reactivity
Reacts with: Human -
Product overview
ab134005 is for simultaneous detection of the relative levels of 17 different Phosphorylated Human EGF Receptors. Suitable for cell and tissue lysates only.
Targets: EGFR (Tyr845), EGFR (Tyr992), EGFR (Tyr1045), EGFR (Tyr1068), EGFR (Tyr1086), EGFR (Tyr1148), EGFR (Tyr1173), EGFR (Ser1046/1047), EGFR (Ser1070), ErbB2 (Tyr877), ErbB2 (Tyr1112), ErbB2 (Tyr 1221/1222), ErbB2 (Tyr1248), ErbB2 (Thr686), ErbB2 (Ser1113), ErbB3 (Tyr1289), ErbB4 (Tyr1284)
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Tested applications
Suitable for: Multiplex Protein Detectionmore details
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 4 Membranes 1 x 8 Membranes 1 x 2 Membranes 1,000X HRP-Conjugated Streptavidin 1 x 18µl 1 x 18µl 1 x 18µl 100X Phosphatase Inhibitor Cocktail Set I Concentrate 1 vial 2 vials 1 vial 1X Blocking Buffer 1 x 25ml 2 x 25ml 1 x 25ml 20X Wash Buffer I 1 x 30ml 1 x 30ml 1 x 30ml 20X Wash Buffer II 1 x 30ml 1 x 30ml 1 x 30ml 2X Cell Lysis Buffer 1 x 5ml 1 x 5ml 1 x 5ml 8-Well Incubation Tray (with Lid) 1 unit 1 unit 1 unit Cocktail of Biotin-Conjugated Anti-EGFR 2 vials 4 vials 1 vial Detection Buffer C 1 x 1.5ml 1 x 2.5ml 1 x 1.5ml Detection Buffer D 1 x 1.5ml 1 x 2.5ml 1 x 1.5ml EGFR Phosphorylation Antibody Array Membranes 4 units 8 units 2 units Phosphatase Inhibitor Cocktail Set II 1 vial 2 vials 1 vial Protease Inhibitor Cocktail 1 vial 2 vials 1 vial -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134005 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Multiplex Protein Detection |
Use at an assay dependent concentration.
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| Notes |
|---|
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Multiplex Protein Detection
Use at an assay dependent concentration. |
Images
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Multiplex Protein Detection - Human EGFR Phosphorylation Antibody Array - Membrane (17 Targets) (ab134005)
Human epidermoid carcinoma cell line, A431 cells that were 80-90% confluent were serum starved overnight, then exposed to 100 ng/ml EGF for 20 minutes at 37°C. Control cells were serum starved without the subsequent stimulation with EGF. Cell lysates were prepared according to the protocol. To use ab134005 Human EGFR Phosphorylation Antibody Array - Membrane, treated or untreated cell lysate was added into antibody array membrane. The antibody array membranes were washed and cocktail of biotinylated anti-EGFR was used to detect phosphorylated proteins on activated receptors. After incubation with HRP-Conjugated Streptavidin, the signals were visualized by chemiluminescence.
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Western blot - Human EGFR Phosphorylation Antibody Array - Membrane (17 Targets) (ab134005)Western blot analysis of extracts from 100 ng/ml hEGF treated A431 cells or untreated A431 cells. Phospho-EGFR (Tyr845) or Phospho-EGFR (Tyr1173) antibodies were used in this assay.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab134005 has been referenced in 3 publications.
- Chen JC et al. Valproic acid-induced amphiregulin secretion confers resistance to temozolomide treatment in human glioma cells. BMC Cancer 19:756 (2019). PubMed: 31370819
- Lee PC et al. Targeting PKCd as a Therapeutic Strategy against Heterogeneous Mechanisms of EGFR Inhibitor Resistance in EGFR-Mutant Lung Cancer. Cancer Cell 34:954-969.e4 (2018). PubMed: 30537515
- Huang TW et al. The role of Thyroid Transcription Factor-1 and Tumor differentiation in Resected Lung Adenocarcinoma. Sci Rep 7:14222 (2017). PubMed: 29079814