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  1. Link

    human-egfr-phosphorylation-antibody-array-membrane-17-targets-ab134005.pdf

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Signal Transduction Growth Factors/Hormones EGF
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Human EGFR Phosphorylation Antibody Array - Membrane (17 Targets) (ab134005)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a question References (3)

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Multiplex Protein Detection - Human EGFR Phosphorylation Antibody Array - Membrane (17 Targets) (ab134005)
  • Western blot - Human EGFR Phosphorylation Antibody Array - Membrane (17 Targets) (ab134005)

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Overview

  • Product name

    Human EGFR Phosphorylation Antibody Array - Membrane (17 Targets)
  • Sample type

    Tissue Extracts, Cell Lysate
  • Assay type

    Semi-quantitative
  • Species reactivity

    Reacts with: Human
  • Product overview

    ab134005 is for simultaneous detection of the relative levels of 17 different Phosphorylated Human EGF Receptors. Suitable for cell and tissue lysates only.


     


    Targets: EGFR (Tyr845), EGFR (Tyr992), EGFR (Tyr1045), EGFR (Tyr1068), EGFR (Tyr1086), EGFR (Tyr1148), EGFR (Tyr1173), EGFR (Ser1046/1047), EGFR (Ser1070), ErbB2 (Tyr877), ErbB2 (Tyr1112), ErbB2 (Tyr 1221/1222), ErbB2 (Tyr1248), ErbB2 (Thr686), ErbB2 (Ser1113), ErbB3 (Tyr1289), ErbB4 (Tyr1284)


    Learn more about membrane antibody arrays

  • Notes

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Tested applications

    Suitable for: Multiplex Protein Detectionmore details

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 4 Membranes 1 x 8 Membranes 1 x 2 Membranes
    1,000X HRP-Conjugated Streptavidin 1 x 18µl 1 x 18µl 1 x 18µl
    100X Phosphatase Inhibitor Cocktail Set I Concentrate 1 vial 2 vials 1 vial
    1X Blocking Buffer 1 x 25ml 2 x 25ml 1 x 25ml
    20X Wash Buffer I 1 x 30ml 1 x 30ml 1 x 30ml
    20X Wash Buffer II 1 x 30ml 1 x 30ml 1 x 30ml
    2X Cell Lysis Buffer 1 x 5ml 1 x 5ml 1 x 5ml
    8-Well Incubation Tray (with Lid) 1 unit 1 unit 1 unit
    Cocktail of Biotin-Conjugated Anti-EGFR 2 vials 4 vials 1 vial
    Detection Buffer C 1 x 1.5ml 1 x 2.5ml 1 x 1.5ml
    Detection Buffer D 1 x 1.5ml 1 x 2.5ml 1 x 1.5ml
    EGFR Phosphorylation Antibody Array Membranes 4 units 8 units 2 units
    Phosphatase Inhibitor Cocktail Set II 1 vial 2 vials 1 vial
    Protease Inhibitor Cocktail 1 vial 2 vials 1 vial
  • Research areas

    • Signal Transduction
    • Growth Factors/Hormones
    • EGF
    • Cancer
    • Signal transduction
    • Protein phosphorylation
    • Tyrosine kinases
    • Receptor tyrosine kinases
    • Antibody Arrays
    • Membrane antibody arrays
    • EGFR phosphorylation antibody arrays

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab134005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Multiplex Protein Detection
Use at an assay dependent concentration.
Notes
Multiplex Protein Detection
Use at an assay dependent concentration.

Images

  • Multiplex Protein Detection - Human EGFR Phosphorylation Antibody Array - Membrane (17 Targets) (ab134005)
    Multiplex Protein Detection - Human EGFR Phosphorylation Antibody Array - Membrane (17 Targets) (ab134005)

    Human epidermoid carcinoma cell line, A431 cells that were 80-90% confluent were serum starved overnight, then exposed to 100 ng/ml EGF for 20 minutes at 37°C. Control cells were serum starved without the subsequent stimulation with EGF. Cell lysates were prepared according to the protocol. To use ab134005 Human EGFR Phosphorylation Antibody Array - Membrane, treated or untreated cell lysate was added into antibody array membrane. The antibody array membranes were washed and cocktail of biotinylated anti-EGFR was used to detect phosphorylated proteins on activated receptors. After incubation with HRP-Conjugated Streptavidin, the signals were visualized by chemiluminescence.

  • Western blot - Human EGFR Phosphorylation Antibody Array - Membrane (17 Targets) (ab134005)
    Western blot - Human EGFR Phosphorylation Antibody Array - Membrane (17 Targets) (ab134005)
    Western blot analysis of extracts from 100 ng/ml hEGF treated A431 cells or untreated A431 cells. Phospho-EGFR (Tyr845) or Phospho-EGFR (Tyr1173) antibodies were used in this assay.

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (3)

Publishing research using ab134005? Please let us know so that we can cite the reference in this datasheet.

ab134005 has been referenced in 3 publications.

  • Chen JC  et al. Valproic acid-induced amphiregulin secretion confers resistance to temozolomide treatment in human glioma cells. BMC Cancer 19:756 (2019). PubMed: 31370819
  • Lee PC  et al. Targeting PKCd as a Therapeutic Strategy against Heterogeneous Mechanisms of EGFR Inhibitor Resistance in EGFR-Mutant Lung Cancer. Cancer Cell 34:954-969.e4 (2018). PubMed: 30537515
  • Huang TW  et al. The role of Thyroid Transcription Factor-1 and Tumor differentiation in Resected Lung Adenocarcinoma. Sci Rep 7:14222 (2017). PubMed: 29079814

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