Human EGFR Phosphorylation Antibody Array - Membrane (17 Targets) (ab134005)

Overview

  • Product name

    Human EGFR Phosphorylation Antibody Array - Membrane (17 Targets)
  • Sample type

    Tissue Extracts, Cell Lysate
  • Assay type

    Semi-quantitative
  • Species reactivity

    Reacts with: Human
  • Product overview

    ab134005 is for simultaneous detection of the relative levels of 17 different Phosphorylated Human EGF Receptors. Suitable for cell and tissue lysates only.


     


    Targets: EGFR (Tyr845), EGFR (Tyr992), EGFR (Tyr1045), EGFR (Tyr1068), EGFR (Tyr1086), EGFR (Tyr1148), EGFR (Tyr1173), EGFR (Ser1046/1047), EGFR (Ser1070), ErbB2 (Tyr877), ErbB2 (Tyr1112), ErbB2 (Tyr 1221/1222), ErbB2 (Tyr1248), ErbB2 (Thr686), ErbB2 (Ser1113), ErbB3 (Tyr1289), ErbB4 (Tyr1284)


    Learn more about membrane antibody arrays

  • Tested applications

    Suitable for: Multiplex Protein Detectionmore details

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 4 Membranes 1 x 8 Membranes 1 x 2 Membranes
    1,000X HRP-Streptavidin Buffer 1 x 18µl 1 x 18µl 1 x 18µl
    100X Phosphatase Inhibitor Cocktail Set I Concentrate 1 vial 2 vials 1 vial
    1X Blocking Buffer 1 x 25ml 2 x 25ml 1 x 25ml
    20X Wash Buffer I 1 x 30ml 1 x 30ml 1 x 30ml
    20X Wash Buffer II 1 x 30ml 1 x 30ml 1 x 30ml
    2X Cell Lysis Buffer 1 x 5ml 1 x 5ml 1 x 5ml
    8-Well Incubation Tray (with Lid) 1 unit 1 unit 1 unit
    Cocktail of Biotin-Conjugated Anti-EGFR 2 vials 4 vials 1 vial
    Detection Buffer C 1 x 1.5ml 1 x 2.5ml 1 x 1.5ml
    Detection Buffer D 1 x 1.5ml 1 x 2.5ml 1 x 1.5ml
    EGFR Phosphorylation Antibody Array Membranes 4 units 8 units 2 units
    Phosphatase Inhibitor Cocktail Set II 1 vial 2 vials 1 vial
    Protease Inhibitor Cocktail 1 vial 2 vials 1 vial
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab134005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Multiplex Protein Detection Use at an assay dependent concentration.

Images

  • Human epidermoid carcinoma cell line, A431 cells that were 80-90% confluent were serum starved overnight, then exposed to 100 ng/ml EGF for 20 minutes at 37°C. Control cells were serum starved without the subsequent stimulation with EGF. Cell lysates were prepared according to the protocol. To use ab134005 Human EGFR Phosphorylation Antibody Array - Membrane, treated or untreated cell lysate was added into antibody array membrane. The antibody array membranes were washed and cocktail of biotinylated anti-EGFR was used to detect phosphorylated proteins on activated receptors. After incubation with HRP-Conjugated Streptavidin, the signals were visualized by chemiluminescence.

  • Western blot analysis of extracts from 100 ng/ml hEGF treated A431 cells or untreated A431 cells. Phospho-EGFR (Tyr845) or Phospho-EGFR (Tyr1173) antibodies were used in this assay.

Protocols

References

This product has been referenced in:

  • Huang TW  et al. The role of Thyroid Transcription Factor-1 and Tumor differentiation in Resected Lung Adenocarcinoma. Sci Rep 7:14222 (2017). Read more (PubMed: 29079814) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Of these components, the only one that we are able to make available is the 2X cell lysis buffer. This has been added to our catalog as ab192896 (link below).



https://www.abcam.com/2x-cell-lysis-buffer-ab192896.html

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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