Human EGFR Phosphorylation Antibody Array - Membrane (17 Targets) (ab134005)


  • Product name

    Human EGFR Phosphorylation Antibody Array - Membrane (17 Targets)
  • Sample type

    Tissue Extracts, Cell Lysate
  • Assay type

  • Species reactivity

    Reacts with: Human
  • Product overview

    ab134005 is for simultaneous detection of the relative levels of 17 different Phosphorylated Human EGF Receptors. Suitable for cell and tissue lysates only.


    Targets: EGFR (Tyr845), EGFR (Tyr992), EGFR (Tyr1045), EGFR (Tyr1068), EGFR (Tyr1086), EGFR (Tyr1148), EGFR (Tyr1173), EGFR (Ser1046/1047), EGFR (Ser1070), ErbB2 (Tyr877), ErbB2 (Tyr1112), ErbB2 (Tyr 1221/1222), ErbB2 (Tyr1248), ErbB2 (Thr686), ErbB2 (Ser1113), ErbB3 (Tyr1289), ErbB4 (Tyr1284)

    Learn more about membrane antibody arrays

  • Tested applications

    Suitable for: Multiplex Protein Detectionmore details


  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 4 Membranes 1 x 8 Membranes 1 x 2 Membranes
    1,000X HRP-Streptavidin Buffer 1 x 18µl 1 x 18µl 1 x 18µl
    100X Phosphatase Inhibitor Cocktail Set I Concentrate 1 vial 2 vials 1 vial
    1X Blocking Buffer 1 x 25ml 2 x 25ml 1 x 25ml
    20X Wash Buffer I 1 x 30ml 1 x 30ml 1 x 30ml
    20X Wash Buffer II 1 x 30ml 1 x 30ml 1 x 30ml
    2X Cell Lysis Buffer 1 x 5ml 1 x 5ml 1 x 5ml
    8-Well Incubation Tray (with Lid) 1 unit 1 unit 1 unit
    Cocktail of Biotin-Conjugated Anti-EGFR 2 vials 4 vials 1 vial
    Detection Buffer C 1 x 1.5ml 1 x 2.5ml 1 x 1.5ml
    Detection Buffer D 1 x 1.5ml 1 x 2.5ml 1 x 1.5ml
    EGFR Phosphorylation Antibody Array Membranes 4 units 8 units 2 units
    Phosphatase Inhibitor Cocktail Set II 1 vial 2 vials 1 vial
    Protease Inhibitor Cocktail 1 vial 2 vials 1 vial
  • Research areas


Our Abpromise guarantee covers the use of ab134005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Multiplex Protein Detection Use at an assay dependent concentration.


  • Human epidermoid carcinoma cell line, A431 cells that were 80-90% confluent were serum starved overnight, then exposed to 100 ng/ml EGF for 20 minutes at 37°C. Control cells were serum starved without the subsequent stimulation with EGF. Cell lysates were prepared according to the protocol. To use ab134005 Human EGFR Phosphorylation Antibody Array - Membrane, treated or untreated cell lysate was added into antibody array membrane. The antibody array membranes were washed and cocktail of biotinylated anti-EGFR was used to detect phosphorylated proteins on activated receptors. After incubation with HRP-Conjugated Streptavidin, the signals were visualized by chemiluminescence.

  • Western blot analysis of extracts from 100 ng/ml hEGF treated A431 cells or untreated A431 cells. Phospho-EGFR (Tyr845) or Phospho-EGFR (Tyr1173) antibodies were used in this assay.



This product has been referenced in:

  • Huang TW  et al. The role of Thyroid Transcription Factor-1 and Tumor differentiation in Resected Lung Adenocarcinoma. Sci Rep 7:14222 (2017). Read more (PubMed: 29079814) »
See 1 Publication for this product

Customer reviews and Q&As


Of these components, the only one that we are able to make available is the 2X cell lysis buffer. This has been added to our catalog as ab192896 (link below).

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