Overview

  • Product name

    Human EMMPRIN ELISA Kit
    See all CD147 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Urine 8 2.5%
    Inter-assay
    Sample n Mean SD CV%
    Urine 3 3%
  • Sample type

    Cell culture supernatant, Saliva, Urine, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    3.88 pg/ml
  • Range

    31.3 pg/ml - 200 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 112 111% - 113%
    Saliva 95 94% - 95%
    Urine 98 97% - 98%
    Serum 97 91% - 102%
    Cell culture media 103 99% - 110%
    Heparin Plasma 115 113% - 117%
    EDTA Plasma 108 107% - 109%
    Citrate Plasma 102 100% - 105%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat, Cow
  • Product overview

    EMMPRIN in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of human EMMPRIN protein in human serum, plasma, urine, saliva, and cell culture supernatant samples.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Sensitivity:
    Samples in Sample Diluent 50BS - 15.3 pg/mL
    Samples in Sample Diluent NS - 3.88 pg/mL

  • Notes

    Extracellular matrix metalloproteinase inducer (EMMPRIN), also known as Basigin (BSG), and CD147 is a 363-amino acid protein encoded by the gene BSG. EMMPRIN is a member of the immunoglobin superfamily, and targets the monocarboxylate transporters SLC16A1, SLC16A3, and SLC16A8 to the plasma membrane. EMMPRIN is the determinant for the Oκ blood group system, and has been demonstrated to be a receptor on red blood cells for the malaria parasite, Plasmodium falciparum. In addition to its metalloproteinase-inducing ability, EMMPRIN is thought to regulate several other functions, including spermatogenesis, embryo implantation, neural network formation, and tumor progression. EMMPRIN also has several ligands including cyclophilin proteins, caveolin-1, and integrins.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab219631 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of EMMPRIN were measured in duplicates, interpolated from the EMMPRIN standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EMMPRIN concentration was determined to be 1,572 pg/mL in neat serum, 1,440 pg/mL in neat plasma (citrate), 1,516 pg/mL in neat plasma (EDTA), and 1,740 pg/mL in neat plasma (heparin).

  • The concentrations of EMMPRIN were measured in duplicates, interpolated from the EMMPRIN standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EMMPRIN concentration was determined to be 634 pg/mL in neat serum, 531 pg/mL in neat plasma (citrate), 563 pg/mL in neat plasma (EDTA), and 599 pg/mL in neat plasma (heparin).

  • The concentrations of EMMPRIN were measured in duplicates, interpolated from the EMMPRIN standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 50%, saliva 100%, and PBMC stimulated culture media 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EMMPRIN concentration was determined to be 3,245 pg/mL in neat urine, 1,135 pg/mL in neat saliva, and 1,111 pg/mL in neat PBMC stimulated culture media.

  • The concentrations of EMMPRIN were measured in duplicates, interpolated from the EMMPRIN standard curves and corrected for sample dilution. Undiluted samples are as follows: saliva 100% and PBMC stimulated culture media 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EMMPRIN concentration was determined to be 69 pg/mL in neat saliva, and 75 pg/mL in neat PBMC stimulated culture media.

  • The concentrations of EMMPRIN were measured in duplicates, interpolated from the EMMPRIN standard curves and corrected for sample dilution. Undiluted samples are as follows: unstimulated PBMC media 100%, stimulated PBMC media 100%, untreated MCF-7 media 100%, and treated MCF-7 media 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EMMPRIN concentration was determined to be 72 pg/mL in neat stimulated PBMC media and 64 pg/mL in neat treated MCF-7 media. The unstimulated PBMC media and untreated MCF-7 media samples measured below the 7th point of the standard curve. PBMC media samples were cultured in RPMI media with 10% fetal bovine serum (unstimulated) for 24 hours and then stimulated for 48 hours with 1.5% PHAM. MCF-7 media samples were cultured in DMEM media with 10% fetal bovine serum (untreated) and then treated with 0.12 mM NEAA with 1 mM sodium pyruvate (treated) for 24 hours.

  • Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EMMPRIN concentration was determined to be 625 pg/mL with a range of 445- 836 pg/mL.

Protocols

References

ab219631 has not yet been referenced specifically in any publications.

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