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    human-ena-screen-elisa-kit-ab178619.pdf

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Human Anti-ENA Screen ELISA Kit (ab178619)

  • Datasheet
  • SDS
  • Protocol Booklet
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Serologic ELISA assay principle

    Key features and details

    • Sensitivity: 3.68 U/ml
    • Sample type: Cit plasma, Hep Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Indirect
    • Reacts with: Human

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    Product image
    Native Human IgG FC fragment protein (ab90285)

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    Overview

    • Product name

      Human Anti-ENA Screen ELISA Kit
    • Detection method

      Colorimetric
    • Precision

      Intra-assay
      Sample n Mean SD CV%
      Overall 32 < 8.2%
      Inter-assay
      Sample n Mean SD CV%
      Overall 2 < 11.2%
    • Sample type

      Serum, Hep Plasma, Cit plasma
    • Assay type

      Indirect
    • Sensitivity

      3.68 U/ml
    • Assay duration

      Multiple steps standard assay
    • Species reactivity

      Reacts with: Human
    • Product overview

      Abcam’s anti-ENA Screen Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate semi-quantitative measurement of IgG class antibodies against extractable nuclear antigens: Sm (Smith), RNP/Sm, Scl-70, SS-A (Ro) (52kDa and 60kDa), SS-B (La), Jo-1, in Human serum or plasma.

      A 96-well plate has been precoated with ENA antigens to bind cognate antibodies. Controls, standards or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized ENA-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of ENA IgG sample captured in plate.

    • Platform

      Microplate

    Properties

    • Storage instructions

      Store at +4°C. Please refer to protocols.
    • Components 1 x 96 tests
      10X Wash Solution 1 x 50ml
      Cover Foil 1 unit
      ENA Anti-IgG HRP Conjugate 1 x 15ml
      ENA Coated Microplate (12 x 8 wells) 1 unit
      ENA Negative Control 1 x 1.2ml
      ENA Positive Control 1 x 1.2ml
      ENA Standard 1 x 1.2ml
      IgG Sample Diluent 1 x 100ml
      Stop Solution 1 x 15ml
      Strip holder 1 unit
      TMB Substrate Solution 1 x 15ml
    • Research areas

      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • Pathogen specific immunoglobulins ELISA kits
    • Relevance

      Extractable nuclear antigens (ENA): Sm (Smith), RNP/Sm, Scl-70, SS-A (Ro) (52kDa and 60kDa), SS-B (La), Jo-1, in Human serum or plasma. ENA is a grouping of antibodies often used to screen for mixed connective tissue disease (MCTD), Sjögren's syndrome and systemic lupus erythematosus and commonly is composed of four tests.

    Associated products

      Images

      • Serologic ELISA assay principle
        Serologic ELISA assay principle
        Specific antigens are coated on the 96-well plate, controls or test samples are added to the well and incubated. The wells are washed to remove any unbound Human anti-antigen antibodies (Ig). A horseradish peroxidase (HRP) labelled anti-Human Ig conjugate is added to the wells. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Human anti-antigen Ig captured on the plate.

      Protocols

      • Protocol Booklet

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (0)

      Publishing research using ab178619? Please let us know so that we can cite the reference in this datasheet.

      ab178619 has not yet been referenced specifically in any publications.

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