Key features and details
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
Product nameHuman Anti-Entamoeba histolytica IgG ELISA Kit
Intra-assay Sample n Mean SD CV% Pos.Serum 7 2.1% Inter-assay Sample n Mean SD CV% Pos.Serum 6 3.9%
Sample typeSerum, Hep Plasma, Cit plasma
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s anti-Entamoeba histolytica IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Entamoeba histolytica in Human serum and plasma.
A 96-well plate has been precoated with Entamoeba histolytica antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a Protein A peroxidase labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Entamoeba histolytica-specific antibodies. TMB is then catalyzed by the Protein A to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Entamoeba histolytica IgG sample captured in plate.
Storage instructionsStore at +4°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Cover foil 1 unit Entamoeba histolytica (IgG) Cut-off Control colored yellow; green cap 1 x 3ml Entamoeba histolytica (IgG) Negative Control colored yellow; blue cap 1 x 2ml Entamoeba histolytica (IgG) Positive Control colored yellow; red cap 1 x 2ml Entamoeba histolytica Coated Microplate (12 x 8 wells) 12 strips of 8 wells 1 unit Entamoeba histolytica Protein A Conjugate colored blue; black cap 1 x 20ml IgG Sample Diluent colored yellow; white cap 1 x 100ml Stop Solution red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml
ab108734 has not yet been referenced specifically in any publications.