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    human-eotaxin-2-elisa-kit-ccl24-ab100509.pdf

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Immunology Innate Immunity Chemokines Beta Chemokines (CC)
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Human Eotaxin 2 ELISA Kit (CCL24) (ab100509)

  • Datasheet
  • SDS
  • Protocol Booklet
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Typical Standard Curve
  • Typical Standard Curve

Key features and details

  • Sensitivity: 2 pg/ml
  • Range: 1.37 pg/ml - 1000 pg/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

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Protein
Recombinant Human Eotaxin 2 protein (ab9834)

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Overview

  • Product name

    Human Eotaxin 2 ELISA Kit (CCL24)
    See all Eotaxin 2 kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 2 pg/ml
  • Range

    1.37 pg/ml - 1000 pg/ml
  • Recovery

    95 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 95.77 82% - 103%
    Serum 94.86 83% - 103%
    Plasma 96.74 85% - 104%
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Eotaxin 2 (CCL24) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Eotaxin 2 in serum, plasma, and cell culture supernatants.


    This assay employs an antibody specific for Human Eotaxin 2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Eotaxin 2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Eotaxin 2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Eotaxin 2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Get results in 90 minutes with Human Eotaxin-2 ELISA Kit (CCL24) (ab179882) from our SimpleStep ELISA® range.

  • Notes

    Optimization may be required with urine samples.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    600X HRP-Streptavidin Concentrate 1 x 200µl
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human Eotaxin 2 2 vials
    Eotaxin 2 Microplate (12 x 8 well strips) 1 unit
    Recombinant Human Eotaxin 2 Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas

    • Immunology
    • Innate Immunity
    • Chemokines
    • Beta Chemokines (CC)
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Cytokines and cytokine receptors ELISA kits
  • Relevance

    Eotaxin 2 is a member of the CC chemokine family, based on the presence of the CC motif and homology with other known CC chemokines. Eotaxin 2 cDNA encodes a 119 amino acid residue precursor protein with a 26 amino acid residue signal peptide that is cleaved to generate a mature protein predicted to contain 93 amino acid residues with an N glycosylation site. Mature human eotaxin 2 has a predicted molecular mass of approximately 10.6 kDa. Compared to other CC chemokines, eotaxin 2 exhibits 40 %, 42 %, and 39 % amino acid identity to MCP3, MIP1 alpha, and eotaxin, respectively. Human CC chemokine eotaxin 2 maps to chromosome 7q11.23. Both eotaxin and eotaxin 2 activate and attract eosinophils and basophils. A receptor for human eotaxin has been identified and found to be the third numbered receptor in the C-C chemokine subfamily of receptors (CCR3. On eosinophils, the effects of eotaxin 2 is inhibited by an CCR3 antibody and cross-desensitized by eotaxin and MCP4, suggesting that all three CC chemokines act through CCR3. Eotaxin 2 mRNA is weakly expressed in activated monocytes and T lymphocytes. Recombinant eotaxin 2 induces chemotaxis of eosinophils, basophils, and resting T lymphocytes but not monocytes and activated T lymphocytes. Eotaxin 2 inhibits colony formation in myleloid progenitor cells.
  • Cellular localization

    Secreted
  • Alternative names

    • C C motif chemokine 24
    • CCL24
    • Chemokine (C C motif) ligand 24
    • Chemokine CC Motif Ligand 2
    • CK beta 6
    • Ckb 6
    • Ckb6
    • Eosinophil chemotactic protein 2
    • Eotaxin 2 like protein
    • MPIF 2
    • MPIF2
    • Myeloid progenitor inhibitory factor 2
    • OTTHUMP00000210225
    • SCYA24
    • Small inducible cytokine A24
    • Small inducible cytokine subfamily A (Cys Cys) member 24
    see all
  • Database links

    • Entrez Gene: 6369 Human
    • Omim: 602495 Human
    • SwissProt: O00175 Human

    Associated products

      Images

      • Typical Standard Curve
        Typical Standard Curve

        Representative Standard Curve using ab100509.

      • Typical Standard Curve
        Typical Standard Curve

        Representative Standard Curve using ab100509.

      Protocols

      • Protocol Booklet

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (1)

      Publishing research using ab100509? Please let us know so that we can cite the reference in this datasheet.

      ab100509 has been referenced in 1 publication.

      • Segal-Salto M  et al. A blocking monoclonal antibody to CCL24 alleviates liver fibrosis and inflammation in experimental models of liver damage. JHEP Rep 2:100064 (2020). PubMed: 32039405

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