Human Epiregulin ELISA Kit (ab213775)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 3.9 pg/ml - 250 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Epiregulin ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 1 16 13pg/ml 0.78 = 6% 2 16 57pg/ml 4.21 = 7.4% 3 16 113pg/ml 6.1 = 4.6% Inter-assay Sample n Mean SD CV% 1 24 12pg/ml 0.94 = 7.9% 2 24 56pg/ml 4.48 = 8% 3 24 105pg/ml 6.4 = 6.1% -
Sample type
Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 10 pg/ml -
Range
3.9 pg/ml - 250 pg/ml -
Assay time
3h 30m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
The Human Epiregulin Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213775) is designed for the quantitative measurement of Human Epiregulin in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Epiregulin has been pre-coated onto 96-well plates. Standards (E.coli; V60-L108) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Epiregulin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Epiregulin amount of sample captured in plate.
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Notes
Epiregulin (EPR) is a protein that in humans is encoded by the EREG gene. It is a member of the epidermal growth factor family. Epiregulin can function as a ligand of epidermal growth factor receptor (EGFR), as well as a ligand of most members of the ERBB (v-erb-b2 oncogene homolog) family of tyrosine-kinase receptors. The secondary structure at the C-terminus epiregulin is different from other epidermal growth factor family ligands because of the lack of hydrogen bonds. The structural difference at the C-terminus may provide an explanation for the reduced binding affinity of epiregulin to the ERBB receptors.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Adhesive Plate Seal 4 units Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Human Epiregulin coated Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti- Human Epiregulin antibody 1 x 100µl Lyophilized recombinant Human Epiregulin Standard 2 vials Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Black Cap 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml Wash Buffer (25X) 1 x 20ml -
Research areas
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Function
May be a mediator of localized cell proliferation. As a mitogen it may stimulate cell proliferation and/or angiogenesis. -
Tissue specificity
In normal adults, expressed predominantly in the placenta and peripheral blood leukocytes. High levels were detected in carcinomas of the bladder, lung, kidney and colon. -
Sequence similarities
Contains 1 EGF-like domain. -
Cellular localization
Cell membrane and Secreted > extracellular space. - Information by UniProt
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Alternative names
- Epiregulin
- EPR
- ER
see all -
Database links
- Entrez Gene: 2069 Human
- Omim: 602061 Human
- SwissProt: O14944 Human
- Unigene: 115263 Human
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab213775 has not yet been referenced specifically in any publications.