Overview

  • Product name

    Human Anti-Epstein Barr virus IgG ELISA Kit (EBV-VCA)
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Pos. Serum 10 2.9%
    Inter-assay
    Sample n Mean SD CV%
    Pos. Serum 15 4.3%
  • Sample type

    Serum, Hep Plasma, Cit plasma
  • Assay type

    Indirect
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s anti-Epstein Barr virus (EBV-VCA) IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Epstein Barr virus in Human serum and plasma.

     

    A 96-well plate has been precoated with Epstein Barr virus antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Epstein Barr virus-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Epstein Barr virus IgG sample captured in plate.

  • Tested applications

    Suitable for: Indirect ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108730 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA Use at an assay dependent concentration.

Protocols

References

This product has been referenced in:

  • Salovin A  et al. Anti-NMDA receptor encephalitis and nonencephalitic HSV-1 infection. Neurol Neuroimmunol Neuroinflamm 5:e458 (2018). Read more (PubMed: 29629396) »
  • Tian Y  et al. Unique phenotypes and clonal expansions of human CD4 effector memory T cells re-expressing CD45RA. Nat Commun 8:1473 (2017). Read more (PubMed: 29133794) »
See all 2 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Anti-VCA antibodies in neurological diseases

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Works well, tested in plasma

Abcam user community

Verified customer

Submitted Sep 26 2019

Question
Answer

Thank you very much for contacting us and for your patience. My colleague Elena is out of the office today, but I'll be happy to help you with this issue.

We have heard back from the lab, and the kit is validated for serum and citrate plasma only. We do not have any information regarding the use of heparin samples so it's unknown whether these are suitable for use with the kit. I am sorry that we don't have more information in this regard.

Please let me know if you have further questions or need anything else.

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Question
Answer

Thank you for contacting us.

The lab let me know the following:

TheEBV VCA IgG ELISA kitis coated with a synthetic p18 peptide.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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