Key features and details
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
Product nameHuman Anti-Epstein Barr virus IgG ELISA Kit (EBV-VCA)
Intra-assay Sample n Mean SD CV% Pos. Serum 10 2.9% Inter-assay Sample n Mean SD CV% Pos. Serum 15 4.3%
Sample typeSerum, Hep Plasma, Cit plasma
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s anti-Epstein Barr virus (EBV-VCA) IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Epstein Barr virus in Human serum and plasma.
A 96-well plate has been precoated with Epstein Barr virus antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Epstein Barr virus-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Epstein Barr virus IgG sample captured in plate.
Storage instructionsStore at +4°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Cover foil 1 unit Epstein Barr virus (IgG) Coated Microplate (12 x 8 wells) 1 unit Epstein Barr virus anti-IgG HRP Conjugate colored blue; black cap 1 x 20ml Epstein-Barr Virus IgG Cut-off Control colored yellow; green cap 1 x 3ml Epstein-Barr Virus IgG Negative Control colored yellow; blue cap 1 x 2ml Epstein-Barr Virus IgG Positive Control colored yellow; red cap 1 x 2ml IgG Sample Diluent colored yellow; white cap 1 x 100ml Stop Solution red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml
Specific antigens are coated on the 96-well plate, controls or test samples are added to the well and incubated. The wells are washed to remove any unbound Human anti-antigen antibodies (Ig). A horseradish peroxidase (HRP) labelled anti-Human Ig conjugate is added to the wells. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Human anti-antigen Ig captured on the plate.
ab108730 has been referenced in 3 publications.
- Kuri A et al. Epidemiology of Epstein-Barr virus infection and infectious mononucleosis in the United Kingdom. BMC Public Health 20:912 (2020). PubMed: 32532296
- Salovin A et al. Anti-NMDA receptor encephalitis and nonencephalitic HSV-1 infection. Neurol Neuroimmunol Neuroinflamm 5:e458 (2018). PubMed: 29629396
- Tian Y et al. Unique phenotypes and clonal expansions of human CD4 effector memory T cells re-expressing CD45RA. Nat Commun 8:1473 (2017). PubMed: 29133794