Human ErbB3 / HER3 ELISA Kit (ab100511)
Key features and details
- Sensitivity: 4 pg/ml
- Range: 3.43 pg/ml - 2500 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human ErbB3 / HER3 ELISA Kit
See all ErbB3 / HER3 kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 4 pg/ml -
Range
3.43 pg/ml - 2500 pg/ml -
Recovery
> 90 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 100.1 78% - 113% Serum 95.12 79% - 111% Plasma 88.87 71% - 106% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s ErbB3 (Epidermal Factor Growth Factor Receptor 3) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human ErbB3 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for human ErbB3 coated on a 96-well plate. Standards and samples are pipetted into the wells and ErbB3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human ErbB3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ErbB3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Notes
Optimisation may be required with urine samples.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human ErbB3 2 vials ErbB3 Microplate (12 x 8 wells) 1 unit Recombinant Human ErbB3 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Binds and is activated by neuregulins and NTAK. -
Tissue specificity
Epithelial tissues and brain. -
Involvement in disease
Defects in ERBB3 are the cause of lethal congenital contracture syndrome type 2 (LCCS2) [MIM:607598]; also called Israeli Bedouin multiple contracture syndrome type A. LCCS2 is an autosomal recessive neurogenic form of a neonatally lethal arthrogryposis that is associated with atrophy of the anterior horn of the spinal cord. The LCCS2 syndrome is characterized by multiple joint contractures, anterior horn atrophy in the spinal cord, and a unique feature of a markedly distended urinary bladder. The phenotype suggests a spinal cord neuropathic etiology. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Contains 1 protein kinase domain. -
Developmental stage
Overexpressed in a subset of human mammary tumors. -
Domain
The cytoplasmic part of the receptor may interact with the SH2 or SH3 domains of many signal-transducing proteins. -
Post-translational
modificationsLigand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Alternative names
- c erbB 3
- c erbB3
- Erb b2 receptor tyrosine kinase 3
see all -
Database links
- Entrez Gene: 2065 Human
- Omim: 190151 Human
- SwissProt: P21860 Human
- Unigene: 118681 Human
Images
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TYPICAL STANDARD CURVE - Assay Diluent A – Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
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TYPICAL STANDARD CURVE - Assay Dilent B – Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab100511 has been referenced in 1 publication.
- D'Abronzo LS et al. Evaluation of Protein Levels of the Receptor Tyrosine Kinase ErbB3 in Serum. Methods Mol Biol 1655:319-334 (2018). PubMed: 28889394