Overview

  • Product name

    Human ERCC1 Antibody Pair - BSA and Azide free
    See all ERCC1 kits
  • Detection method

    Colorimetric
  • Assay type

    ELISA set
  • Range

    125 pg/ml - 8000 pg/ml
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Antibody Pair can be used to quantify Human ERCC1. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.


    For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab210582), which uses the same antibodies. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.


    To receive an electronic copy of the Certificate of Analysis, please send an email to technical support with "CoA for matched antibody pair kit" in the subject line and the desired product number and lot number in the body of the email.


    Download SDS here.


     

  • Tested applications

    Suitable for: ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 10 x 96 tests
    Human ERCC1 Capture Antibody 1 x 100µg
    Human ERCC1 Detector Antibody 1 x 100µg
  • Research areas

  • Function

    Structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair.
  • Involvement in disease

    Defects in ERCC1 are the cause of cerebro-oculo-facio-skeletal syndrome type 4 (COFS4) [MIM:610758]. COFS is a degenerative autosomal recessive disorder of prenatal onset affecting the brain, eye and spinal cord. After birth, it leads to brain atrophy, hypoplasia of the corpus callosum, hypotonia, cataracts, microcornea, optic atrophy, progressive joint contractures and growth failure. Facial dysmorphism is a constant feature. Abnormalities of the skull, eyes, limbs, heart and kidney also occur.
  • Sequence similarities

    Belongs to the ERCC1/RAD10/SWI10 family.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Alternative names

    • COFS 4
    • COFS4
    • DNA excision repair protein ERCC 1
    • DNA excision repair protein ERCC-1
    • DNA excision repair protein ERCC1
    • ERCC 1
    • ERCC1
    • ERCC1_HUMAN
    • Excision repair cross complementation group 1
    • Excision repair cross complementing 1
    • Excision Repair Cross Complementing Rodent Repair Deficiency Complementation Group 1
    • Excision repair protein
    • RAD 10
    • RAD10
    • UV 20
    • UV20
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab241812 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

References

ab241812 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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