Overview

  • Product name

    Human Erythropoietin ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 5 4.8%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 3.3%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    2.8 mU/ml
  • Range

    15.41 mU/ml - 116 mU/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 105.8 101.5% - 110.8%
    Serum 97.7 91.5% - 102.4%
    Heparin Plasma 100.6 94.3% - 108.2%
    EDTA Plasma 98.7 93.2% - 102.1%
    Citrate Plasma 81.5 78.2% - 84.5%

  • Assay time

    2h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat
  • Product overview

    Erythropoietin (EPO) in vitro  ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Erythropoietin protein in human serum, plasma and cell culture supernatant samples.


    The ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material.  TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Sensitivity:
    Samples diluted in Sample Diluent NS MDD = 2.8 mU/mL.
    Samples diluted in Sample Diluent 10BS MDD = 3.4 mU/mL.
    Samples diluted in Sample Diluent 30BS MDD = 4.9 mU/mL.

  • Notes

    Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass. It is a secreted protein produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals. Erythropoietin is used for the treatment of anemia. Variations affecting the Erythropoietin gene are associated with microvascular complications of diabetes 2 (MVCD2) [MIM: 612623].

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab211647 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of Erythropoietin were measured in duplicates, interpolated from the Erythropoietin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Erythropoietin concentration was determined to be 43.8 mU/mL in neat serum.

  • The concentrations of Erythropoietin were measured in duplicates, interpolated from the Erythropoietin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100%, cell culture media containing 10% bovine serum 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • The concentrations of Erythropoietin were measured in duplicates, interpolated from the Erythropoietin standard curves and corrected for sample dilution. Undiluted samples are as follows: plasma (citrate) 50%, plasma (EDTA) 100%, plasma (heparin) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Erythropoietin concentration was determined to be 21.3 mU/mL with a range of not detectable – 198.4 mU/mL. Note that not detectable donor # 2, and donor # 7 and 9 were excluded from the mean value.

Protocols

References

ab211647 has not yet been referenced specifically in any publications.

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