Key features and details
- Sensitivity: 0.4 mIU/ml
- Range: 1.6 mIU/ml - 100 mIU/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman Erythropoietin ELISA Kit (EPO)
See all Erythropoietin (EPO) kits
Intra-assay Sample n Mean SD CV% Overall 8 3.4% Inter-assay Sample n Mean SD CV% Overall 8 6.2%
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Range1.6 mIU/ml - 100 mIU/ml
Sample specific recovery Sample type Average % Range Serum 81 71% - 93%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s Erythropoietin (EPO) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human Erythropoietin concentrations in cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Erythropoietin specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotinylated Erythropoietin detection antibody and the microplate is then incubated at room temperature. Following washing with wash buffer a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and unbound conjugates are then washed away using was buffer. TMB is then added and catalyzed by HRP to produce a blue color product that changes to yellow after addition of an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Erythropoietin captured in plate.
Abcam is committed to meeting high quality standards of ethical manufacturing and has decided to discontinue this product by March 2021 as it contains antibodies generated by the ascites method. We are sorry for any inconvenience this may cause. We suggest ab274397 as a possible replacement.
Storage instructionsPlease refer to protocols.
Components 1 x 96 tests 20X Assay Buffer Concentrate 1 x 5ml 20X Wash Buffer Concentrate 1 x 50ml Adhesive Films 4 units Biotin-Conjugate anti-human Erythropoietin (EPO) monoclonal antibody 1 x 70µl EPO Standard lyophilized (200 mIU/mL upon reconstitution) 2 vials High control, lyophilized 1 vial Low control, lyophilized 1 vial Microplate coated with monoclonal antibody to EPO (12 x 8 wells) 1 unit Sample Diluent 1 x 12ml Stop Solution (1M Phosphoric acid) 1 x 15ml Streptavidin-HRP 1 x 150µl TMB Substrate Solution 1 x 15ml
FunctionErythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass.
Tissue specificityProduced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.
Involvement in diseaseGenetic variation in EPO is associated with susceptbility to microvascular complications of diabetes type 2 (MVCD2) [MIM:612623]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
Sequence similaritiesBelongs to the EPO/TPO family.
- Information by UniProt
ab119522 has been referenced in 3 publications.
- Olmos G et al. Impaired erythropoietin synthesis in chronic kidney disease is caused by alterations in extracellular matrix composition. J Cell Mol Med 22:302-314 (2018). PubMed: 28857467
- Sundem L et al. Erythropoietin Enhanced Recovery After Traumatic Nerve Injury: Myelination and Localized Effects. J Hand Surg Am 41:999-1010 (2016). PubMed: 27593486
- Hansen HG et al. Versatile microscale screening platform for improving recombinant protein productivity in Chinese hamster ovary cells. Sci Rep 5:18016 (2015). WB . PubMed: 26657798