Key features and details
- Purity: > 95% SDS-PAGE
- Suitable for: Blocking
Product nameHuman Estrogen Receptor alpha peptide
See all Estrogen Receptor alpha proteins and peptides
Purity> 95 % SDS-PAGE.
Amino acids21 to 32
Our Abpromise guarantee covers the use of ab5847 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Blocking - Blocking peptide for Anti-Estrogen Receptor alpha antibody (ab3575)
This peptide may be used for neutralization and control experiments with the polyclonal antibody that reacts with this product and Human estrogen receptor a, catalog ab3575. Using a solution with equal weights per unit volume of peptide and corresponding antibody will yield a solution with a large molar excess of peptide that is able to competitively bind the antibody.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
ReconstitutionReconstitute with 0.1 ml of distilled water.
- 7*/654 isoform
- 7*/819 2 isoform
- 7*/822 isoform
FunctionNuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.
Sequence similaritiesBelongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
modificationsPhosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
Glycosylated; contains N-acetylglucosamine, probably O-linked.
Ubiquitinated. Deubiquitinated by OTUB1.
Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
Palmitoylated (isoform 3). Not biotinylated (isoform 3).
Cellular localizationNucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab5847 has been referenced in 2 publications.
- Oshimo Y et al. Estrogen increases KISS1 expression in newly generated immortalized KISS1-expressing cell line derived from goat preoptic area. J Reprod Dev 67:15-23 (2021). PubMed: 33100283
- Sun T et al. Anxiolytic Effects of 8-O-Acetyl Shanzhiside Methylester on Acute and Chronic Anxiety via Inflammatory Response Inhibition and Excitatory/Inhibitory Transmission Imbalance. Neurotox Res N/A:N/A (2020). PubMed: 32367473