Overview

  • Product name

    Human Exodus 2 ELISA Kit (CCL21)
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 3 3.4%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 5.5%
  • Sample type

    Cell culture supernatant, Serum, Heparin Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    3.8 pg/ml
  • Range

    9.4 pg/ml - 600 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 104 102% - 106%
    Cell culture media 99 94% - 105%
    Heparin Plasma 102 99% - 106%
    Citrate Plasma 99 93% - 106%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Exodus 2 in vitro SimpleStep ELISA™ (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Exodus 2 protein in Human serum, plasma and cell culture supernatant samples.


    The SimpleStep ELISA™ employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    Exodus 2 (CCL 21) is a small cytokine in the CC chemokine family, and is involved in inhibition of hemopoiesis and stimulation of chemotaxis. It interacts with atypical chemokine receptor ACKR4 and recruits beta-arrestin to ACKR4.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human Exodus 2 Capture Antibody 1 x 600µl
    10X Human Exodus 2 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml
    Human Exodus 2 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Relevance

    Exodus 2 is one of several CC cytokine genes clustered on the p arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterised by two adjacent cysteines. Similar to other chemokines the protein encoded by this gene inhibits hemopoiesis and stimulates chemotaxis. This protein is chemotactic in vitro for thymocytes and activated T cells, but not for B cells, macrophages, or neutrophils. The cytokine encoded by this gene may also play a role in mediating homing of lymphocytes to secondary lymphoid organs. It is a high affinity functional ligand for chemokine receptor 7 (CCR7) that is expressed on T and B lymphocytes and a known receptor for another member of the cytokine family (small inducible cytokine A19).
  • Alternative names

    • 6Ckine
    • Beta chemokine exodus 2
    • C-C motif chemokine 21
    • Chemokine (C-C motif) ligand 21
    • CKb9
    • ECL
    • Efficient Chemoattractant for Lymphocytes
    • Exodus2
    • MGC34555
    • OTTHUMP00000000526
    • OTTHUMP00000000527
    • OTTHUMP00000021303
    • SCYA 21
    • SCYA21
    • Secondary lymphoid tissue chemokine
    • SLC
    • Small inducible cytokine A21
    • Small inducible cytokine subfamily A (Cys-Cys), member 21
    • TCA 4
    • TCA4
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab193759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD, n = 3) are graphed

  • Mean Human Exodus 2 values fall within expected normal reference ranges (WHO).

  • Mean Human Exodus 2 is 479 pg/mL and values fall within expected normal reference ranges (WHO).

Protocols

References

This product has been referenced in:

  • Suh KJ  et al. EGFR or HER2 inhibition modulates the tumor microenvironment by suppression of PD-L1 and cytokines release. Oncotarget 8:63901-63910 (2017). ELISA . Read more (PubMed: 28969039) »
  • Yamashita M  et al. Lymphangiogenic factors are associated with the severity of hypersensitivity pneumonitis. BMJ Open Respir Res 2:e000085 (2015). ELISA ; Human . Read more (PubMed: 26448865) »
See all 2 Publications for this product

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