Overview

  • Product name

    Human FABP-1 ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 4.9%
    Inter-assay
    Sample n Mean SD CV%
    Overall 9.4%
  • Sample type

    Cell culture supernatant, Saliva, Milk, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma, Cerebral Spinal Fluid
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    1 ng/ml
  • Range

    1.563 ng/ml - 50 ng/ml
  • Assay time

    4h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
    Does not react with: Rabbit, Cow
  • Product overview

    Human FABP-1 ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab222875) is designed for the quantitative measurement of human FABP-1 protein in plasma, serum, saliva, milk, cerebral spinal fluid and cell culture samples.


    The assay employs a quantitative sandwich enzyme immunoassay technique that measures human FABP-1 in approximately 4 hours. A polyclonal antibody specific for human FABP-1 has been pre-coated onto a 96-well microplate with removable strips. FABP-1 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human FABP-1, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added.  The color development is stopped and the intensity of the color is measured.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Notes

    Mitochondrial glutamate oxaloacetate transaminase 2 (FABP-1), also called mitochondrial aspartate aminotransferase (mAspAT) and fatty acid-binding protein, belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. GOT is a pyridoxal phosphate-dependent enzyme, which exists in cytoplasmic GOT1 and inner-membrane mitochondrial FABP-1 forms. GOT plays a role in amino acid metabolism and in the urea and tricarboxylic acid cycles. The two enzymes show close homology. FABP-1 is a dimer containing two identical subunits, each 45 kDa with about 401 amino acids. FABP-1 acetylation stimulates the malate–aspartate NADH shuttle activity and oxidative protection. It is also involved in cell proliferation and energy production.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    1X Standard Diluent 1 x 2ml
    20X Wash Buffer Concentrate 2 x 30ml
    30X Biotinylated Human FABP-1 1 x 180µl
    Anti-Human FABP-1 coated Microplate (12 x 8 wells) 1 unit
    Chromogen Substrate 1 x 8ml
    Human FABP-1 Standard  1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • Research areas

  • Function

    Plays a key role in amino acid metabolism. Important for metabolite exchange between mitochondria and cytosol. Facilitates cellular uptake of long-chain free fatty acids.
  • Sequence similarities

    Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.
  • Cellular localization

    Mitochondrion matrix. Cell membrane. Exposure to alcohol promotes translocation to the cell membrane.
  • Information by UniProt
  • Alternative names

    • AATM_HUMAN
    • AL022787
    • Aspartate aminotransferase
    • Aspartate aminotransferase 2
    • Aspartate aminotransferase, mitochondrial
    • Aspartate transaminase 2
    • ASPATA
    • EC 2.6.1.1
    • FABP 1
    • FABP pm
    • FABP-1
    • FABPpm
    • Fatty acid binding protein
    • Fatty acid-binding protein
    • FLJ40994
    • Glutamate oxaloacetate transaminase 2
    • Glutamate oxaloacetate transaminase 2, mitochondrial
    • Glutamate oxaloacetate transaminase, mitochondrial
    • Glutamic oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2)
    • Got 2
    • GOT2
    • KAT4
    • KATIV
    • kynurenine aminotransferase 4
    • Kynurenine aminotransferase IV
    • kynurenine--oxoglutarate transaminase 4
    • kynurenine--oxoglutarate transaminase IV
    • mAspAT
    • MGC102129
    • MGC115763
    • mitAAT
    • mitochondrial
    • Mitochondrial aspartate aminotransferase
    • OTTMUSP00000017748
    • Plasma membrane fatty acid binding protein
    • Plasma membrane-associated fatty acid-binding protein
    • Transaminase A
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab222875 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Example of human FABP-1 standard curve. Background-subtracted data are graphed.

Protocols

References

ab222875 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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