Overview

  • Product name

    Human FABP2 ELISA Kit
    See all FABP2 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 9 1.8%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 3.3%
  • Sample type

    Cell culture supernatant, Urine, Serum, Cell culture extracts, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    1.7 pg/ml
  • Range

    12.5 pg/ml - 800 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Urine 83 82% - 85%
    Serum 119 111% - 125%
    Cell culture extracts 95 92% - 97%
    Cell culture media 100 98% - 102%
    Heparin Plasma 108 103% - 110%
    EDTA Plasma 115 109% - 120%
    Citrate Plasma 113 105% - 118%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat, Cow
  • Product overview

    FABP2 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of FABP2 protein in human serum, plasma, urine, cell culture supernatant and cell extracts.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB Development Solution is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Intestinal Fatty acid-binding protein, also known as Fatty acid-binding protein 2 (FABP2), is a 132- amino acid protein encoded by the FABP2 gene and is a member of the calycin superfamily. FABP2 is involved in the uptake, metabolism, and transport of long-chain fatty acids. FABP2 preferentially binds sixteen to twenty carbon chain saturated fatty acids but will bind with less affinity to unsaturated fatty acids. FABP2 participates in the processing of fatty acids into triglycerides by trafficking the fatty acids from the plasma membrane to the endoplasmic reticulum. FABP2 is generally found at low levels in serum at normal physiological conditions, and higher circulating levels may indicate intestinal damage.


    Sensitivity:


    Samples diluted in Sample Diluent NS - 1.7 pg/mL


    Samples diluted in 1X Cell Extraction Buffer PTR - 3.2 pg/mL

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Wash Buffer PT (ab206977) 1 x 20ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 4BR 1 x 6ml
    10X Human FABP2 Capture Antibody 1 x 600µl
    10X Human FABP2 Detector Antibody 1 x 600µl
    Human FABP2 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Function

    FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor.
  • Tissue specificity

    Expressed in the small intestine and at much lower levels in the large intestine. Highest expression levels in the jejunum.
  • Sequence similarities

    Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
  • Domain

    Forms a beta-barrel structure that accommodates the hydrophobic ligand in its interior.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Alternative names

    • FABP2
    • FABPI_HUMAN
    • Fatty acid-binding protein
    • Fatty acid-binding protein 2
    • I-FABP
    • intestinal
    • Intestinal-type fatty acid-binding protein
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab234566 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of FABP2 were measured in duplicates, interpolated from the FABP2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (heparin) 50%, plasma (EDTA) 25%, plasma (citrate) 50%, and LoVo extract 750 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FABP2 concentration in the neat samples was determined to be 1,248 pg/mL in serum, 1,104 pg/mL in plasma (heparin), 1,642 pg/mL in plasma (EDTA), and 1,199 pg/mL in plasma (citrate).

  • The concentrations of FABP2 were measured in duplicate and interpolated from the FABP2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FABP2 concentration was determined to be 1,094 pg/mL with a range of 422 – 3,639 pg/mL.

  • Standard curve comparison between human FABP2 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

Protocols

References

ab234566 has not yet been referenced specifically in any publications.

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